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US 6,475,759 B1Granted
Low PH lactic acid fermentation
AssigneeCargill, Incorporated
InventorsTing Liu Carlson, Eugene Max Peters, Jr.
Life of this patent
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Abstract
A process for producing lactic acid which includes incubating acid-tolerant homolactic bacteria in nutrient medium to produce a fermentation broth with high levels of free lactic acid is provided. An isolated acid-tolerant homolactic bacteria capable of producing high levels of free lactic acid is also provided.
Claims17
A process for producing lactic acid comprising:
- (a) incubating an acid-tolerant homolactic bacterial strain in nutrient medium at an incubation pH sufficient to allow production of lactic acid by the acid-tolerant homolactic bacterial stra in the nutrient medium, wherein the final incubation pH is pH 4.0 or less, and the step of incubating is provided at a temperature of at least about 30° C. to produce:
- (i) a solution including at least about 25 g/L free L-lactic acid or at least about 25 g/L free D-lactic acid;
- (ii) free lactic acid at an overall rate of at least about 1.0 g/L/hr;
- and
- (iii) the solution including at least about 25 g/L free L-lactic acid or at least about 25 g/L free D-lactic acid, wherein the free L-lactic acid is provided at an optical purity of at least about 50% or the free D-lactic acid is provided at an optical purity of at least about 50%;
- and
- (b) recovering free lactic acid.
4 more elements
A process for producing lactic acid comprising:
- (a) incubating an acid-tolerant homolactic bacterial strain in nutrient medium comprising corn steep water at an incubation pH sufficient to allow production of lactic acid by the acid-tolerant homolactic bacterial strain in the nutrient medium, wherein the final incubation pH is pH 4.2 or less, and the step of incubating is provided at a temperature of at least about 30° C. to produce:
- (i) a solution having at least about 25 g/L free L-lactic acid or at least about 25 g/L free D-lactic acid;
- and
- (ii) the solution having at least about 25 g/L free L-lactic acid or at least about 25 g/L free D-lactic acid, wherein the free L-lactic acid is provided at an optical purity of at least about 80% or the free D-lactic acid is provided at an optical purity of at least about 80%;
- (b) recovering free lactic acid.
2 more elements
Dependencies are inferred from the claim text.