Polyhydroxyalkanoate synthase genes
AssigneePioneer Hi-Bred International, Inc.
InventorsMatthias Liebergesell, Patrica Lynne Fallis, Jian G. Dong, Chun Ping Li, Scott E. Nichols
Life of this patent
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Abstract
The invention relates to genes encoding polyhydroxyalkanoate synthases. Compositions and methods for producing polyhydroxyalkanoate are provided. Such compositions and methods find use in producing biodegradable thermoplastics in host cells and transgenic plants. Isolated nucleotide molecules, isolated polypeptides, expression cassettes and genetically manipulated host cells, plants, plant tissues, plant cells and seeds are also provided.
Claims35
An isolated nucleotide molecule comprising a member selected from the group consisting of:
- (a) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NO:
- 1 or 3;
- (b) a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2 or 4;
- and
- (c) a nucleotide sequence fully complementary to at least one nucleotide sequence selected from the group consisting of the nucleotide sequences of (a) and (b);
- wherein said member encodes a polypeptide comprising PHA syhthase activity or said member is complementary to a nucleotide sequence that encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence having at least 85% sequence identity to at least one nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence having at least 90% sequence identity to at least one nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence having at least 95% sequence identity to at least one nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence having at least 75 contiguous bases of at least one member selected from the group consisting of:
- the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, and a nucleotide sequence that is fully complementary to the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity or said nucleotide molecule is complementary to a nucleotide sequence that encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence having at least 80% sequence identity to at least one member selected from the group consisting of:
- the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, and a nucleotide sequence that is fully complementary to the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity or said nucleotide molecule is complementary to a nucleotide sequence that encodes a polypeptide comprising PHA synthase activity.
An isolated nucleotide molecule comprising a nucleotide sequence that hybridizes under stringent conditions to at least one member selected from the group consisting of:
- the nucleotide sequences set forth in SEQ ID NO:
- 1 and SEQ ID NO:
- 3, and a nucleotide sequence that is fully complementary to the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- wherein said stringent conditions comprise at least one post-hybridization wash at a temperature of about 55° C. to about 60° C. in a solution comprising about 0.5× to about 1×SSC, and wherein said nucleotide molecule encodes a polypeptide comprising PHA synthase activity or said nucleotide molecule is complementary to a nucleotide sequence that encodes a polypeptide comprising PHA synthase activity.
Dependencies are inferred from the claim text.