Primers and methods for the detection of disseminated tumor cells
AssigneeMicromet Gesellschaft Fur Biomedizinische Forschung mbH
InventorsPeter Kufer, Alfred Zippelius
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Abstract
The present invention relates to primers that specifically hybridize to nucleic acid molecules complementary to the messenger RNA transcribed from genes encoding MAGE tumor-specific antigens or a part thereof or to a complementary strand thereof encoding MAGE tumor specific antigens as well as to diagnostic compositions comprising said antigens. The present invention further relates to methods for detecting disseminated tumor cells employing the primers of the invention as well as methods for preparing a tumor adjuvant vaccine.
Claims20
A primer which specifically hybridizes to a nucleic acid molecule complementary to a messenger RNA transcribed from a gene encoding a MAGE tumor-specific antigen or a fragment thereof or to a complementary strand thereof, said primer being selected from primers having at least 15 contiguous 3′-end nucleotides as set forth in the sequences provided in one of the following groups of primers:
- (a) 5′-gtagagttcggccgaaggaac-3′ (SEQ ID NO:
- 1) 5′-caggagctgggcaatgaagac-3′ (SEQ ID NO:
- 2) 5′-cattgaaggagaagatctgcct-3′ (SEQ ID NO:
- 3) 5′-gagtagaagaggaagaagcggt-3′ (SEQ ID NO:
- 4) 5′-gaagccggcccaggctcg-3′ (SEQ ID NO:
- 5) 5′-gatgactctggtcagggcaa-3′ (SEQ ID NO:
- 6) 5′-caccaaggagaagatctgcct-3′ (SEQ ID NO:
- 7) 5′-tcctcagtagtaggagcctgt-3′ (SEQ ID NO:
- 8) 5′-tccgtgaggaggcaaggttc-3′ (SEQ ID NO:
- 9) 5′-atcggattgactccagagagta-3′ (SEQ ID NO:
- 10);
- or (b) 5′-tagagttcggccgaaggaac-3′ (SEQ ID NO:
- 11) 5′-ctgggcaatgaagacccaca-3′ (SEQ ID NO:
- 12) 5′-cattgaaggagaagatctgcct-3′ (SEQ ID NO:
- 13) 5′-caggcttgcagtgctgactc-3′ (SEQ ID NO:
- 14) 5′-ggctcggtgaggaggcaag-3′ (SEQ ID NO:
- 15) 5′-gatgactctggtcagggcaa-3′ (SEQ ID NO:
- 16) 5′-caccaaggagaagatctgcct-3′ (SEQ ID NO:
- 17) 5′-caggcttgcagtgctgactct-3′ (SEQ ID NO:
- 18) 5′-tccgtgaggaggcaaggttc-3′ (SEQ ID NO:
- 19) 5′-gagcctgcgcacccaccaa-3′ (SEQ ID NO:
- 20).
A primer capable of specifically hybridizing to a nucleic acid molecule complementary to the messenger RNA transcribed from a gene encoding a MAGE tumor-specific antigen or a part thereof or to a complementary strand thereof, said nucleic acid molecule or complementary strand thereof having a sequence selected from:
- 5′-gttccttcggccgaactctac-3′ (SEQ ID NO:
- 21);
- 5′-gtcttcattgcccagctcctg-3′ (SEQ ID NO:
- 22);
- 5′-aggcagagatcttctccttcaatg-3′ (SEQ ID NO:
- 23);
- 5′-accgcttcttcctcttctactc-3′ (SEQ ID NO:
- 24);
- 5′-cgagcctgggccggcttc-3′ (SEQ ID NO:
- 25);
- 5′-ttgccctgaccagagtcatc-3′ (SEQ ID NO:
- 26);
- 5′-aggcagatcttctccttggtg-3′ (SEQ ID NO:
- 27);
- 5′-acaggctcctactactgagga-3′ (SEQ ID NO:
- 28);
- 5′-gaaccttgcctcctcacgga-3′ (SEQ ID NO:
- 29);
- 5′-tactctctggagtcaatccgat-3′ (SEQ ID NO:
- 30);
- 5′-gttccttcggccgaactcta-3′ (SEQ ID NO:
- 31);
- 5′-tgtgggtcttcattgcccag-3′ (SEQ ID NO:
- 32);
- 5′-aggcagatcttctccttcaatg-3′ (SEQ ID NO:
- 33);
- 5′-gagtcagcactgcaagcctg-3′ (SEQ ID NO:
- 34);
- 5′-cttgcctcctcaccgagcc-3′ (SEQ ID NO:
- 35);
- 5′-ttgccctgaccagagtcatc-3′ (SEQ ID NO:
- 36);
- 5′-aggcagatcttctccttggtg-3′ (SEQ ID NO:
- 37);
- 5′-agagtcagcactgcaagcctg-3′ (SEQ ID NO:
- 38);
- 5′-gaaccttgcctcctcacgga-3′ (SEQ ID NO:
- 39);
- and 5′-ttggtgggtgcgcaggctc-3′ (SEQ ID NO :40).
Dependencies are inferred from the claim text.