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US 6,475,717 B1Granted
Method for detecting and determining mediators
AssigneeDade Behring Marburg GmbH
InventorsKarlheinz Enssle, Roland Kurrle, Klaus-Dieter Langner, Leander Lauffer, Josef-Urban Pauly, Friedrich-Robert Seiler
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Abstract
The invention relates to a method for detecting and determining mediators and/or their derivatives in fluids, the mediator being detected directly or indirectly with the aid of a recombinant, soluble receptor for the mediator to be detected.
Claims21
A method for determining an analyte in a sample of a liquid medium comprising:
- i) bringing the sample into contact with
- (a) a soluble, recombinant receptor, or derivative thereof, for the analyte, and
- (b) a specific antibody directed against the analyte, to form a receptor-analyte-antibody complex;
- ii) detecting said receptor-analyte-antibody complex;
- and
- iii) determining the amount of analyte from the amount of said receptor-analyte-antibody complex;
- wherein the specific antibody directed against the analyte is bound to a solid phase.
4 more elements
A method for detecting a protein analyte in a sample of a liquid medium comprising:
- i) bringing the sample into contact with
- (a) a soluble, recombinant receptor, or a derivative thereof, for the protein analyte, and
- (b) a specific antibody directed against the protein analyte, to form a receptor-protein analyte-antibody complex;
- and
- ii) detecting said receptor-protein analyte-antibody complex;
- wherein the specific antibody directed against the protein analyte is bound to a solid phase.
3 more elements
Dependencies are inferred from the claim text.