Method for preparing closed vesicles
AssigneeMitsubishi Chemical Corporation
InventorsToshiaki Tagawa, Saiko Hosokawa, Kazuhiro Nagaike
Life of this patent
Estimating the term…
Abstract
A method for preparing a closed vesicle by a rehydration of a closed vesicle using a rehydration solution, wherein the closed vesicle comprises a dehydrated micelle particle or dehydrated amphipathic micelle bilayers, preferably a liposome loading a pharmaceutically active substance, and the method is characterized in that the rehydration is carried out at a low temperature such as in the range of from 0° C. to 10° C. The method can provide a rehydrated stable vesicle without a leak of the loaded substance.
Claims15
A method for reducing leakage of loaded substance in the form of a solution, encapsulated in a closed vesicle, which method comprises dehydrating loaded micelle particles or loaded amphipathic micelle bilayers to form dehydrated micelle particles or dehydrated amphipathic micelle bilayers and then rehydrating said dehydrated micelle particles or amphipathic micelle bilayers by using a rehydration solution, wherein said rehydration is carried out in the presence of a saccharide in an outer aqueous phase of said closed vesicle at a temperature of about 20° C. or lower, and said closed vesicle before being rehydrated containing no internal saccharide therein, to reduce leakage of the loaded substance.
Dependencies are inferred from the claim text.