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US 6,472,537 B1Granted
Polyamides for binding in the minor groove of double stranded DNA
AssigneeCalifornia Institute of Technology
InventorsEldon E. Baird, Peter B. Dervan
Life of this patent
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Abstract
The invention encompasses improved polyamides for binding to specific nucleotide sequences in the minor groove of double stranded DNA. The 3-hydroxy-N-methylpyrrole/N-methylpyrrole carboxamide pair specifically recognizes the T·A base pair, while the N-methylpyrrole/3-hydroxy-N-methylpyrrole pair recognizes A·T nucleotide pairs. Similarly, an N-methylimidizole/N-methylpyrrole carboxamide pair specifically recognizes the G·C nucleotide pair, and the N-methylpyrrole/N-methylimidizole carboxamide pair recognizes the C·G nucleotide pair.
Claims17
A polyamide comprising:
- at least three consecutive carboxamide pairs that bind in a sequence-specific manner to an equal number of consecutive DNA base pairs in a duplex DNA sequence, at least one of which consecutive DNA base pairs is an A·T or T·A DNA base pair, wherein said polyamide comprises a 3-hydroxy-N-methylpyrrole (“Hp”)/N-methylpyrrole (“Py”) carboxamide pair to bind to each T·A base pair in said consecutive DNA base pairs and a Py/Hp carboxamide pair to bind to each A·T DNA base pair in said consecutive DNA base pairs.
Dependencies are inferred from the claim text.