US 6,472,228 B2 · Composition and methods for synthesis of novel tracers for detecting amphetamine and methamphetamine in samples

Abstract

This invention relates to novel tracers and their synthesis and use in an immunoassay for the detection of controlled drugs such as amphetamine (APM), methamphetamine (MAPM) and their derivatives, in a biological or aqueous sample. In particular, this invention provides methods for synthesizing novel tracers in which a non-controlled substance is both the starting material in tracer synthesis and the binding site on the resulting novel tracer for the antibody, thereby eliminating the necessity of using controlled substances as starting materials. In addition, the novel tracers of the present invention can be used as an analyte analog in an immunoassay, such as a continuous flow displacement immunoassay. It was unexpectedly discovered that the novel tracers of the present invention substantially improve the performance of the continuous flow displacement immunoassay as compared with conventionally designed tracers.

Claims28

Dependent claims

A synthetic tracer for use in an immunoassay for detecting the presence and or quantity of amphetamine and its derivatives in a sample of the formula:

wherein
(a) n is a number selected from the group consisting of 2, 3 and 4;
(b) R is selected from the group consisting of —(CH2)n— and C(O)—(CH2)nCO;
(c) X is selected from the group consisting of O, NH, CO, HNCSNH, CH2, S, and SO2;
(d) Z is selected from the group consisting of an amino group or a carboxyl group;
(e) * is a label attached to Z.

3 more elements

Dependencies are inferred from the claim text.

Composition and methods for synthesis of novel tracers for detecting amphetamine and methamphetamine in samples

Life of this patent

Composition and methods for synthesis of novel tracers for detecting amphetamine and methamphetamine in samples

Life of this patent

Abstract

Claims28