Methods and compositions for genetically modifying primate bone marrow cells
AssigneeIntrogene B.V.
InventorsDomenico Valerio, Victor Willem Van Beusechem
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Abstract
A method is provided for genetically modifying primate bone marrow cells, comprising isolating bone marrow cells from a primate and, by means which enhance the local concentration of retroviral contacting the isolated bone marrow cells to cells that produce a recombinant amphotropic retrovirus with a genome based on a retroviral vector that contains the genetic information to be introduced into the bone marrow cells. Recombinant amphotropic retrovirus-producing cells, suitable for use in this method also are provided, as are genetically modified primate bone marrow cells with the capacity for regeneration in vivo.
Claims14
A method for preparing genetically modified primate hematopoietic stem cells, said method comprising:
- a) providing isolated primate hematopoietic stem cells,
- b) co-culturing the isolated primate hematopoietic stem cells with retrovirus producer cells which produce a recombinant retroviral vector, wherein the retroviral vector contains a DNA sequence of interest,
- c) harvesting from the co-culture both non-adherent and adherent primate hematopoietic stem cells which contain the DNA sequence of interest.
A method for preparing genetically modified primate hematopoietic cells, said method comprising:
- a) providing isolated primate hematopoietic cells,
- b) co-culturing the isolated primate hematopoietic cells with retrovirus producer cells which produce a recombinant retroviral vector, wherein the retroviral vector contains a DNA sequence of interest,
- c) harvesting from the co-culture both non-adherent and adherent primate hematopoietic cells which contain the DNA sequence of interest.
Dependencies are inferred from the claim text.