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US 6,472,208 B1Granted
Method of producing human IFN-α using sendai virus-infected hematopoietic stem cells
AssigneesHéma-Québec, Canadian Blood Services
InventorsRéal Lemieux, Sonia Néron, Chantal Proulx
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Abstract
The present invention relates to a novel method for producing human natural interferon-α using ex vivo expanded cord blood hematopoietic cells infected with sendaivirus, on a large scale.
Claims5
A process for producing ex vivo natural interferon α (nIFN-α), said method comprising the steps of:
- a) expanding under suitable conditions hematopoietic stem cells (HSCs) in culture;
- b) priming said HSCs for nIFN-α secretion by incubating said HSCs in IFN-α;
- c) infecting said hematopoietic stem cells (HSCs) in culture medium with Sendai virus;
- and
- d) culturing the HSCs of step c) for a time sufficient for said HSCs to produce natural IFN-α,
- wherein said HSCs produce more natural IFN-α when expanded according to step a), than HSCs that have not been expanded.
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Dependencies are inferred from the claim text.