Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation
AssigneeCanon Kabushiki Kaisha
InventorsTetsuya Yano, Tsuyoshi Nomoto, Takeshi Imamura
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Abstract
A recombinant DNA is constructed by using a toluene monooxygenase gene isolated from Ralstonia eutropha strain TB64 and employed to provide the transformant which can express toluene monooxygenase useful for cleaning of aqueous media such as drain and waste water containing halogenated aliphatic hydrocarbon compounds or aromatic compounds, for remediation of soil polluted with such compounds, and cleaning of air (gas phase) polluted with volatile organic chlorine compounds.
Claims19
An isolated DNA fragment of about 5.3 Kb containing a toluene monooxygenase gene, having 3 BamHI, 1 C1aI, 1 EcoRI, 3 KpnI, 2 NcoI, 2 NspV, 2 ScaI, 2 SmaI, 2 SphI, 1 StuI, 0 DraI, 0 EcoRV, 0 HindIII, OHpaI, 0 NdeI, 0 PvuII, 0 ScaI, 0 Sse83871, 0 XbaI, 0 XhoI restriction sites, and having a restriction map of:
An isolated DNA fragment having a nucleotide sequence of SEQ ID NO:
- 1 in the Sequence Listing.
A DNA fragment encoding a protein having a toluene monooxygenase activity and being hybridizable to a nucleotide sequence from 200 . . . 4799 of SEQ ID NO:
- 1 or a complementary sequence thereof under stringent hybridization conditions.
Dependencies are inferred from the claim text.