Biocatalytic synthesis of galloid organics
AssigneeBoard of Trustees operating Michigan State Univerisity
InventorJohn W. Frost
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Abstract
The present invention provides a bioengineered synthesis scheme for the production of gallic acid from a carbon source. Methods of producing gallic acid from a carbon source based on the synthesis scheme are also provided. The gallic acid produces from these methods can be further converted to pyrogallol. Methods for the biosynthesis of pyrogallol from gallic acid are also provided.
Claims22
A method for the production of gallic acid from a carbon source, comprising:
- a) providing a host cell which is capable of converting the carbon source to 3-dehydroshikimic acid and comprising a recombinant DNA encoding an enzyme which converts 3-dehydroshikimic acid to protocatechuic acid and a recombinant DNA encoding an enzyme which converts protocatechuic acid to gallic acid;
- and
- b) culturing the host cell in the presence of the carbon source.
A method for producing gallic acid from a carbon source comprising culturing, in the presence of the carbon source, a cell which has been transformed by introducing into the cell (a) a recombinant DNA encoding an enzyme which converts 3-dehydroshikimic acid to protocatechuic acid and (b) a recombinant DNA encoding an enzyme which converts protocatechuic acid to gallic acid.
A method for the production of gallic acid from a carbon source comprising converting the carbon source to gallic acid with a recombinant E. coli comprising the genes encoding an isozyme of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase insensitive to feedback inhibition by aromatic amino acids or other aromatic molecules, a 3-dehydroquinate synthase, a 3-dehydroshikimate dehydratase, and a mutant p-hydroxybenzoate hydroxylase capable of catalyzing the hydroxylation of protocatechuic acid to gallic acid.
Dependencies are inferred from the claim text.