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US 6,472,181 B1Granted
Hybrid matrix implants and explants
AssigneeTranskaryotic Therapies, Inc.
InventorRochelle Mineau-Hanschke
Life of this patent
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Abstract
An implantable device having a body of matrix material made up of insoluble collagen fibrils, and disposed therewithin (a) a plurality of vertebrate cells; and (b) a plurality of microspheres each of which consists primarily of one or more of the following materials: collagen, polystyrene, dextran, polyacrylamide, cellulose, calcium alginate, latex, polysulfone, or glass.
Claims20
A method of producing a polypeptide in vitro, comprising:
- providing a composition comprising a body of matrix material comprising insoluble collagen fibrils, there being embedded within the body of matrix material (a) a plurality of cultured vertebrate cells which express the polypeptide and (b) a plurality of microspheres;
- maintaining the composition under conditions whereby the cultured vertebrate cells express and secrete the polypeptide;
- contacting the composition with a liquid such that the cells secrete the polypeptide into the liquid;
- and
- obtaining the polypeptide from the liquid.
2 more elements
Dependencies are inferred from the claim text.