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US 6,472,169 B1Granted
Biocatalytic synthesis of shikimic acid
AssigneeBoard of Trustees operating Michigan State University
InventorsJohn W. Frost, Karen M. Frost, David R. Knop
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Abstract
A bioengineered synthesis scheme for the production of shikimic acid from a carbon source is provided. Methods of producing shikimic acid from a carbon source based on the synthesis scheme are also provided.
Claims21
A method for the production of shikimic acid from a carbon source comprising:
- a) providing a microbe selected from the group consisting of bacteria and fungi including yeast, wherein the microbe comprises one or more enzyme-encoding recombinant DNA molecules, wherein the encoded enzyme is selected from the group consisting of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and 3-dehydroshikimate dehydrogenase, and wherein the microbe further comprises an inactivating mutation of at least one DNA molecule which encodes a shikimate kinase isozyme, and wherein the microbe further comprises an inactivating mutation in at least one DNA sequence which encodes a gene involved in the phosphoenolpyruvate carbohydrate phosphotransferase system;
- and
- b) culturing the microbe in the presence of the carbon source;
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- c) isolating the shikimic acid produced.
2 more elements
Dependencies are inferred from the claim text.