Method for preparing thrombin for use in a biological glue

A method for extracting and then dispensing thrombin, the steps consisting of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol, wherein ethanol is present at a concentration between about 8% and about 20% volume per unit volume,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe, and
• using the syringe to dispense the thrombin to stem blood flow.

A method for extracting thrombin from one person, the steps consisting of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a concentration between about 8% and about 20% volume per unit volume,
• converting the prothrombin to thrombin, and
• sequestering the thrombin.

A method for extracting autologous thrombin from a patient, the steps consisting essentially of:

• obtaining a blood product from the patient;
• sequestering plasma from the blood product;
• adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a concentration between about 8% and about 20% volume per unit volume;
• converting the prothrombin in the solution to thrombin;
• filtering the thrombin to remove particulate matter;
• and
• applying the thrombin to the patient.

A method for extracting and then dispensing thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol, wherein ethanol is present at a concentration between about 8% and about 20% volume per unit volume,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe, and
• using the syringe to dispense the thrombin to stem blood flow.

A method for extracting thrombin from one person, the steps consisting essentially of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a concentration between about 8% and about 20% per volume per unit volume,
• converting the prothrombin to thrombin, and
• sequestering the thrombin.

A method for extracting thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a concentration between about 8% and about 20% per volume per unit volume,
• converting the prothrombin to thrombin, and
• sequestering the thrombin.

A method for extracting and then dispensing thrombin, the steps consisting of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• making the thrombin stable for a period of time between about 15 minutes and about 360 minutes,
• loading the thrombin into a syringe, and
• the syringe to dispense the thrombin to stem blood flow.

A method for extracting thrombin from one person, the steps consisting of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• making the thrombin stable for a period of time between about 15 minutes and about 360 minutes, and
• sequestering the thrombin.

A method for extracting and then dispensing thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• making the thrombin stable for a period of time between about 15 minutes and about 360 minutes,
• loading the thrombin into a syringe, and
• using the syringe to dispense the thrombin to stem blood flow.

A method for generating thrombin from one person, the steps consisting essentially of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• making the thrombin stable for a period of time between about 15 minutes and about 360 minutes, and
• sequestering the thrombin.

A method for extracting thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• making the thrombin stable for a period of time between about 15 minutes and about 360 minutes, and
• sequestering the thrombin.

A method for extracting autologous thrombin from a patient, the steps consisting of:

• obtaining a blood product from the patient;
• sequestering plasma from the blood product;
• adding ethanol to the plasma to prepare a solution containing prothrombin;
• converting the prothrombin in the solution to thrombin;
• filtering the thrombin to remove particulate matter;
• and
• applying the thrombin to the patient,
• wherein the time required to extract the autologous thrombin is between about 30 minutes and about 75 minutes.

A method for extracting and then dispensing thrombin, the steps consisting of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe, and
• using the syringe to dispense the thrombin to stem blood flow,
• wherein the time required to extract the thrombin is between about 30 minutes and about 75 minutes.

A method for extracting thrombin from one person, the steps consisting of:

• adding ethanol to sequester prothrombin from plasma taken from one person,
• converting the prothrombin to thrombin, and
• removing particulate material from the thrombin,
• wherein the time required to extract the thrombin is between about 30 minutes and about 75 minutes.

A method for extracting thrombin from one person, the steps consisting of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin;
• and
• sequestering the thrombin,
• wherein the time required to extract the thrombin is between about 30 minutes and about 75 minutes.

A method for extracting autologous thrombin from a patient, the steps consisting essentially of:

• obtaining a blood product from the patient;
• sequestering plasma from the blood product;
• adding ethanol to the plasma to prepare a solution containing prothrombin;
• converting the prothrombin in the solution to thrombin;
• filtering the thrombin to remove particulate matter;
• and
• applying the thrombin to the patient,
• wherein the time required to extract the autologous thrombin is between about 30 minutes and about 75 minutes.

A method for extracting and then dispensing thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe, and
• using the syringe to dispense the thrombin to stem blood flow,
• wherein the time required to extract the thrombin is between about 30 minutes and about 5 minutes.

A method for extracting thrombin from one person, the steps consisting essentially of:

• adding ethanol to sequester prothrombin from plasma taken from one person,
• converting the prothrombin to thrombin, and
• removing particulate material from the thrombin,
• wherein the time required to extract the thrombin is between about 30 minutes and about 75 minutes.

A method for extracting thrombin from one person, the steps consisting essentially of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin, and sequestering the thrombin,
• wherein the time required to extract the thrombin is between about 30 minutes and about 75 minutes.

A method for extracting thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin, and sequestering the thrombin.

A method for extracting autologous thrombin from a patient, the steps consisting of:

• obtaining a blood product from the patient;
• sequestering plasma from the blood product;
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin in the solution to thrombin;
• filtering the thrombin to remove particulate matter;
• applying the thrombin to the patient;
• and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting and then dispensing thrombin, the steps consisting of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe,
• using the syringe to dispense the thrombin to stem blood flow, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting thrombin from one person, the steps consisting of:

• adding ethanol to sequester prothrombin from plasma taken from one person,
• converting the prothrombin to thrombin,
• removing particulate material from the thrombin, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting thrombin from one person, the steps consisting of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• sequestering the thrombin, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting autologous thrombin from a patient, the steps consisting essentially of:

• obtaining a blood product from the patient;
• sequestering plasma from the blood product;
• adding ethanol to the plasma to prepare a solution containing prothrombin;
• converting the prothrombin in the solution to thrombin;
• filtering the thrombin to remove particulate matter;
• applying the thrombin to the patient;
• and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting and then dispensing thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• sequestering prothrombin from the whole blood by addition of ethanol,
• converting the prothrombin to thrombin,
• loading the thrombin into a syringe,
• using the syringe to dispense the thrombin to stem blood flow, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting thrombin from one person, the steps consisting essentially of:

• adding ethanol to sequester prothrombin from plasma taken from one person,
• converting the prothrombin to thrombin,
• removing particulate material from the thrombin, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting thrombin from one person, the steps consisting essentially of:

• taking whole blood from one person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• sequestering the thrombin, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.

A method for extracting thrombin, the steps consisting essentially of:

• taking whole blood from a person,
• obtaining plasma from the whole blood,
• adding ethanol to the plasma to prepare a solution containing prothrombin,
• converting the prothrombin to thrombin,
• sequestering the thrombin, and
• mixing the thrombin with clotting proteins to form a fibrin clot, but first altering time required for the thrombin to convert fibrinogen to a fibrin clot to a time of between about two seconds and about five seconds by adding an altering agent selected from the group including calcium ion sources, CaCl2, saline, and sterile water.