Method of evaluating blood clot lysis condition
OwnerRobert F. Baughinventor
Life of this patent
Estimating the term…
Abstract
Blood is tested for clot lysis conditions such as natural lytic capabilities, the effect of previously administered thrombolytic and anti-thrombolytic agents, and dose responses thereto, by forming a clot in a sample of blood, lysing the clot, and measuring the elapsed time period from initial clot formation to clot lysis, all while continuously evaluating the blood sample. Thrombolytic agents include streptokinase, urokinase and recombinant tissue plasminogen activator. Plasmin and plasminogen activator inhibitors, clot activating agents (e.g. kaolin), and agents to deactivate anticoagulants (e.g. heparinase) may also be used as reagents during testing.
Claims31
A method of evaluating a clot lysis condition by measuring lytic activity in blood, comprising the steps of:
- mixing a blood sample with a reagent to induce formation of a clot in the sample of blood;
- continuously evaluating a property of the sample of blood from before a first time when the clot is detected until a second time when lysis of the clot is detected;
- detecting said first time at which said clot forms;
- detecting said second time at which said clot lyses;
- measuring an elapsed time period from said first time at which said clot forms to said second time at which said clot is lysed;
- and
- comparing said elapsed time period with a normal lytic response time to evaluate the clot lysis condition in the sample of blood.
A test cell, in which to perform a clot lysis condition test, said test cell comprising:
- a reagent chamber containing a clot activating agent and one of a thrombolytic or anti-thrombolytic agent;
- a reaction chamber;
- and
- a displaceable sealing member between the reagent and reaction chambers.
A method of evaluating a clot lysis condition in blood, comprising the steps of:
- mixing a blood sample with a reagent to induce formation of a clot in the sample of blood;
- continuously evaluating a property of the sample of blood from a predetermined time before the formation of the clot is detected until lysis of the clot is detected;
- detecting a first time at which said clot forms, wherein said first time is detected by a first change in the property of the sample;
- detecting a second time at which said clot lyses, wherein said second time is detected by a second change in the property of the sample;
- measuring a clot formation time period from said predetermined time to said first time at which said clot is formed;
- measuring a clot lysis time period from said predetermined time to said second time at which said clot is lysed;
- and
- comparing said clot formation and clot lysis times to evaluate the clot lysis condition in the blood.
Dependencies are inferred from the claim text.