US 6,472,148 B1 · Molecular recognition at surfaces derivatized with self-assembled monolayers

Abstract

An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.

Claims14

Dependent claims

A composition comprising a self-assembled monolayer-forming species wherein at least one of said self-assembled monolayer forming species has a formula X—R—NA—NAB, wherein NA represents a nucleic acid strand and NAB represents a biological binding partner of NA.

Dependencies are inferred from the claim text.

Facts

Application

08/786,187Filed 21 Jan 1997 · continuation in part

Priority

26 Sep 1994

Granted

29 Oct 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12Q 1/6837
Enzymatic or biochemical coupling of nucleic acids to a solid phase: using probe arrays or probe chips
B82Y 15/00
Nanotechnology for interacting, sensing or actuating
B82Y 30/00
Nanotechnology for materials or surface science
B82Y 40/00
Manufacture or treatment of nanostructures

Molecular recognition at surfaces derivatized with self-assembled monolayers

Life of this patent

Molecular recognition at surfaces derivatized with self-assembled monolayers

Life of this patent

Abstract

Claims14