Methods for display of heterodimeric proteins on filamentous phage using pVII and pIX, compositions, vectors and combinatorial libraries
AssigneeThe Scripps Research Institute
InventorsKim D. Janda, Peter Wirsching, Richard A. Lerner, Changshou Gao
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Abstract
The invention describes the display of exogenous polypeptides on filamentous phage using a fusion between the exogenous polypeptide and phage pVII or pIX proteins. In particular, phage particles and phagemid vectors are described for expression and display of heterodimeric proteins such a antibody Fv heterodimers in combinatorial libraries, and uses thereof.
Claims5
A filamentous phage encapsulating a genome encoding a fusion polypeptide and having said fusion polypeptide on a surface of said phage, wherein said polypeptide comprises an exogenous polypeptide fused to an amino terminus of a filamentous phage pVII or pIX protein.
Dependencies are inferred from the claim text.