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US 6,471,860 B1Granted
Magnetic micro separation column and method of using it
AssigneeMiltenyi Biotech GmbH
InventorsStefan Miltenyi, Gregor Siebenkotten, Mathias Koester
Life of this patent
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Abstract
A micro column system is provided for high gradient magnetic field separation of macromolecules and/or cells. The system provides fast kinetics and high efficiency as well as the purity and simplicity of a column separation. A yoke provides a magnetic field to a plurality of micro columns. A separation and release process for purifying biological material on the column includes release of the biological material from magnetic particles and elution from the column while the magnetic particles are still magnetically retained by the matrix inside the column.
Claims46
An HGOMS separation column comprising:
- first and second tubular portions, wherein said first and second tubular portions have substantially constant cross-sectional areas, said first tubular portion being integral with said second tubular portion and located above said second tubular portion, said first tubular portion having a first cross-sectional area and said second tubular portion having a second cross-sectional area, said first cross sectional area being larger than said second cross sectional area;
- and
- a matrix adapted to selectively remove at least one component of a mixture as the mixture flows through said tubular portions, wherein said matrix is contained in at least part of said first tubular portions and at least part of said second tubular portion, and wherein said matrix comprises ferromagnetic material.
A separation column comprising:
- first and second tubular portions, said first portion being integral with said second portion and located above said second portion, wherein said first and second portions have substantially constant cross-sectional areas;
- and
- a matrix adapted to selectively remove at least one component of a mixture as the mixture flows through said tubular portions, wherein said matrix is contained in at least part of said first portion and at least part of said second portion, and wherein an amount of said matrix contained in said first portion accomplishes a greater removal function than an amount of said matrix contained in saint second portion.
A column system for high gradient magnetic field separation, comprising:
- a separation unit including a magnetic yoke having at least one notch formed along a length thereof and a pair of magnets placed within each of said at least one notch to from a gap therebetween;
- and
- at least one separation column, each comprising:
- first and second tubular portions, said first portion being integral with said second portion and located above said second portion, wherein said first and second tubular portions have substantially constant cross-sectional areas, and a matrix adapted to selectively remove at least one component of a mixture as the mixture flows through said tubular portions, wherein said matrix is contained in at least part of said first portion and at least part of said second portion, and wherein an amount of said matrix contained in said first portion accomplishes a greater removal function than an amount of said matrix contained in said second portion.
1 more element
A process for purifying biological material on a column, comprising:
- retaining magnetic carriers bound to the biological material with ferromagnetic particles in a magnetic field, wherein said magnetic particles are retained in a matrix contained in a separation column having first and second tubular portions integrally connected, wherein the first tubular is located above the second tubular portion and the first tubular portion has a cross sectional area that is larger than a cross sectional area of the second tubular portion, wherein said first and second cross-sectional areas are substantially constant along the length of each respective tubular portion, and wherein the matrix is contained in at least part of the first tubular portion and at least part of the second tubular portion;
- and eluting the biological material by dissociating the biological material from the magnetic carriers while still in said magnetic field.
A separation column comprising:
- first and second tubular portions, said first portion being integral with said second portion and located above said second portion, wherein said first and second portions have substantially constant cross-sectional areas;
- and
- a matrix adapted to selectively remove at least one component of a mixture as the mixture flows through said tubular portions, wherein said matrix is contained in at least part of said first portion and at least part of said second portion, and wherein an amount of said matrix contained in said second portion accomplishes a greater flow resistance function than an amount of said matrix contained in said first portion.
Dependencies are inferred from the claim text.