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US 6,468,798 B1Granted
Expression of cloned genes in the lung by aerosol and liposome-based delivery
AssigneeThe Regents of the University of California
InventorsRobert James Debs, Ning Zhu
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Abstract
Methods and compositions for producing a mammal capable of expressing an exogenously supplied gene in cells of the airway are disclosed. Liposome-nucleic acid complexes are prepared then delivered via aerosol to the lung airway. The invention provides a direct method for transforming pulmonary cells, treat disorders of the lung, and for delivering substances systematically following expression in the lung.
Claims38
A method of making an aerosolized lipid-nucleic acid complex composition, comprising:
- mixing a cationic liposome and a selected nucleic acid to form a cationic lipid-nucleic acid complex, which complex does not substantially aggregate in vitro;
- and,
- aerosolizing the cationic lipid-nucleic acid complex to provide an aerosolized complex.
Dependencies are inferred from the claim text.