Back to results
US 6,468,759 B1Granted
Direct colorimetric detection of biocatalysts
AssigneeThe Regents of the University of California
InventorDeborah Charych
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions for the direct detection of membrane conformational changes through the detection of color changes in biopolymeric materials. In particular, the present invention allows for the direct colorimetric detection of membrane modifying reactions and analytes responsible for such modifications and for the screening of reaction inhibitors.
Claims16
A method for detecting an enzymatic reaction, comprising:
- a) providing:
- i) a biopolymeric material comprising an enzyme substrate and a plurality of polymerized self-assembling monomers, and
- ii) an enzyme that enzymatically modifies said enzyme substrate such that said enzymatic modification of said enzyme substrate causes a conformational change in said biopolymeric material;
- b) exposing said enzyme said biopolymeric material comprising said enzyme substrate such that said conformational change in said biopolymeric material is produced resulting in a color change of said biopolymeric material;
- and
- c) detecting said color change in said biopolymeric material, wherein said color change indicates at least a partial occurrence of said enzymatic reaction.
3 more elements
Dependencies are inferred from the claim text.