Anthraquinone and its derivatives
AssigneeBiostatus Limited
InventorsPaul James Smith, Laurence Hylton Patterson
Life of this patent
Estimating the term…
Abstract
There is disclosed a compound of formula (I) wherein each of X1 and X2 are independently NH—A—NR1R2, and wherein A is A C2-8 alkylene group and R1 and R2 are independently selected from hydrogen, C1-4 alkyl, C2-4 hydroxy-alkyl and C2-4 aminoalkyl, or R1 and R2 together form a C2-6 alkylene group which with the nitrogen atom to which R1 and R2 are attached forms a heterocyclic ring, or an N-oxide derivative thereof, and wherein the compound (I) or its N-oxide derivative is optionally in the form of an acid salt derived from an organic or inorganic acid. Also disclosed is a method of its production and its uses, including its use in analyzing a cell or biological material and detecting the emitted fluorescence signal.
Claims17
A fluorescent complex comprising a nucleic acid and a compound of the following formula:
- wherein each of X1 and X2 are independently NH—A—NR1R2, and wherein A is a C2-8 alkylene group and R1 and R2 are independently selected from hydrogen, C1-4 alkyl, C2-4 hydroxyalkyl and C2-4 aminoalkyl, or R1 and R2 together form a C2-6 alkylene group which with the nitrogren atom to which R1 and R2 are attached forms a heterocyclic ring,
- and wherein the compound (I) is optionally in the form of an acid salt derived from an organic or inorganic acid.
A method of analysing a cell or biological material containing nucleic acid, comprising the steps of:
- a) preparing a biologically compatible solution containing a compound of the formula (I):
- wherein each of X1 and X2 are independently NH—A—NR1R2, and wherein A is a C2-8 alkylene group and R1 and R2 are independently selected from hydrogen, C1-4 alkyl, C2-4 hydroxyalkyl and C2-4 aminoalkyl, or R1 and R2 together form a C2-6 alkylene group which with the nitrogen atom to which R1 and R2 are attached forms a heterocyclic ring,
- and wherein the compound (I) is optionally in the form of an acid salt derived from an organic or inorganic acid;
- b) treating the cell or biological material with the biologically compatible solution;
- c) exciting the compound (I) in the treated cell or biological material with a light source;
- and
- d) detecting the emitted fluorescence signal.
A method of forming a DNA dye complex comprising combining a compound of the following formula:
- wherein each of X1 and X2 are independently NH—A—NR1R2 and wherein A is a C2-8 alkylene group and R1 and R2 are independently selected from hydrogen, C1-4 alkyl, C2-4 hydroxyalkyl and C2-4 aminoalkyl, or R1 and R2 together form a C2-6 alkylene group which with the nitrogen atom to which R1 and R2 are attached forms a heterocyclic ring,
- and wherein the compound (I) is optionally in the form of an acid salt derived from an organic or inorganic acid;
- with a nucleic acid;
- wherein a stable intercalated complex with DNA is formed with said compound.
Dependencies are inferred from the claim text.