US 6,468,741 B1 · Electrochemiluminescent rhenium moieties

Abstract

A labeled substance comprising a biological substance linked to a luminescent rhenium-containing label. Qualitative and quantitative electrochemiluminescent assays using the same. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

Claims16

Dependent claims

A labeled substance having the formula

[Re(P)(L1)3(L2)]t(B)u
wherein
P is a bidentate ligand of Re selected from the group consisting of bipyridine, substituted bipyridine, bipyrazine, substituted bipyrazine, phenanthroline and substituted phenanthroline;
L1 is a monovalent ligand of Re and is carbon monoxide;
L2 is a monovalent ligand of Re selected from the group consisting of carbon monoxide, cyanide, nitrites, isocyanides, halides, phosphines, arsines, stibine, and nitrogen-containing heterocycles;
B is a biological substance and is conjugated to one or more of P, L1 and L2;
and t and u are integers equal to or greater than one;
P, L1, L2 and B being of such composition and number that the labeled substance can be induced to luminesce.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/157,788Filed 21 Sep 1998 · continuation

Priority

30 Apr 1986

Granted

22 Oct 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

C07D 213/22
Containing only hydrogen and carbon atoms in addition to the ring nitrogen atom: containing two or more pyridine rings directly linked together,…
C07D 213/48
Oxygen atoms: aldehydo radicals
C07D 213/55
Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals:…
C07D 401/12
Containing two hetero rings: linked by a chain containing hetero atoms as chain links

Family

Application06/858,354Parent of this continuation · filed 30 Apr 1986

Application07/117,017Parent of this continuation · filed 4 Nov 1987

Application07/533,931Parent of this continuation · filed 5 Jun 1990

Application08/227,898Parent of this division · filed 15 Apr 1994

Application08/468,524Parent of this continuation · filed 6 Jun 1995

US 5,811,236 AParent of this continuation

Application08/468,524Parent of this division

US 5,591,581 AParent of this division

Application08/227,898Parent of this continuation

US 6,468,741 B1This patent

From the US filing history. Foreign family members aren’t listed.

Electrochemiluminescent rhenium moieties

Life of this patent

Electrochemiluminescent rhenium moieties

Life of this patent

Abstract

Claims16