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US 6,465,253 B1Granted
Vectors and methods for gene transfer to cells
AssigneeGenVec, Inc.
InventorsThomas J. Wickham, Imre Kovesdi, Douglas E. Brough
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AssigneeGenVec, Inc.
InventorsThomas J. Wickham, Imre Kovesdi, Douglas E. Brough
Estimating the term…
The present invention provides a chimeric adenovirus coat protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence. Such a chimeric adenovirus coat protein according to the invention is able to direct entry into cells of a vector comprising the coat protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus coat protein rather than the chimeric adenovirus coat protein. The chimeric coat protein preferably is a fiber, hexon, or penton protein. The present invention also provides an adenoviral vector that comprises the chimeric adenovirus coat protein, as well as methods of constructing and using such a vector.
A chimeric adenovirus coat protein comprising a nonnative amino acid sequence inserted into or in place of an internal coat protein sequence, wherein said chimeric adenovirus coat protein efficiently binds to a broader range of eukaryotic cells than a wild-type adenovirus coat protein and wherein said chimeric adenovirus coat protein is not selective for a specific type of eukaryotic cell.
A chimeric adenovirus coat protein comprising a nonnative amino acid sequence, wherein said chimeric adenovirus coat protein binds to HA SMC cells, HuVec cells, CPAE cells, HS 68 cells, MRC-5 cells, U118 cells, and THP-1 cells more efficiently than a wild-type serotype 5 coat protein.
A chimeric adenovirus coat protein comprising a nonnative amino acid sequence inserted into or in place of an internal coat protein sequence, wherein said chimeric adenovirus coat protein efficiently binds to an epithelial cell, a smooth muscle cell, an endothelial cell, a fibroblast cell, a glioblastoma cell, and a monocyte cell.
A chimeric adenovirus coat protein comprising a nonnative amino acid sequence inserted into or in place of an internal coat protein sequence, wherein said chimeric adenovirus efficiently binds to the majority of all eukaryotic cells.
A chimeric adenovirus coat protein comprising a nonnative amino acid sequence, wherein said chimeric adenovirus coat protein efficiently binds to a moiety present on the surface of the majority of all eukaryotic cells.
Dependencies are inferred from the claim text.