Nucleic acid molecule encoding a cephalosporin acetylesterase
AssigneeBiochemie Gesellschaft m.b.H.
InventorsFranz Knauseder, Martin Schiestl, Kurt Schörgendorfer
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Abstract
The present invention relates to a nucleic acid molecule which codes for the cephalosporin acetylesterase from Bacillus subtilis ATCC 6633 (DSM 11909), vectors and host cells which comprise such a nucleic acid molecule, a process for the recombinant preparation of cephalosporin acetylesterase from B. subtilis ATCC 6633 (DSM 1 1909) using said nucleic acid molecule, and a process for preparing 3-deacetylcephalosporin compounds.
Claims9
A nucleic acid molecule which codes for the cephalosporin acetylesterase from B subtilis ATCC 6633 (DSM 11909), wherein the cephalosporin acetylesterase has the amino acid sequence shown in SEQ ID NO:
- 1.
A process for the production of a 3-deacetylcephalosporin compound, comprising
- (i) converting a cephalosporin compound which has an acetoxymethyl group in position 3 using a recombinantly prepared cephalosporin acetylesterase from B. subtilis ATCC 6633 (DSM 11909), wherein the cephalosporin acetylesterase has the amino acid sequence shown in SEQ ID NO:
- 1 and which is employed in free form or in immobilized form;
- and therafter
- (ii) isolating the 3-deacetylcephalosporin compound formed if desired.
Dependencies are inferred from the claim text.