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US 6,462,258 B1Granted
Plant expression constructs
AssigneeMonsanto Technology LLC
InventorsKaren L. Fincher, Jack Q. Wilkinson
Life of this patent
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Abstract
The present invention relates to a novel plant expression construct. More specifically the present invention provides a DNA construct comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.
Claims5
A DNA construct comprising:
- a first expression cassette, a second expression cassette, and a third expression cassette, wherein said first expression cassette in operable linkage comprises (i) a Figwort mosaic virus 35S promoter;
- (ii) a heterologous 5′ untranslated leader;
- (iii) a chloroplast transit peptide DNA sequence;
- (iv) a DNA sequence encoding a glyphosate tolerant EPSPS;
- and (v) a transcriptional terminator;
- and said second expression cassette comprising in operable linkage (a) an Arabidopsis Elongation factor 1α promoter with homologous EF1α intron;
- (b) a chloroplast transit peptide DNA sequence;
- (d) a DNA sequence encoding a glyphosate tolerant EPSPS;
- and (e) a transcriptional terminator;
- and said third expression cassette comprising in operable linkage (I) an Arabidopsis Act2 promoter with homologous Act2 intron;
- (II) a chloroplast transit peptide DNA sequence;
- (III) a DNA sequence encoding a glyphosate tolerant EPSPS;
- and (IV) a transcriptional terminator.
10 more elements
Dependencies are inferred from the claim text.