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US 6,461,867 B1Granted
Synthetic antigen presenting matrix
AssigneeThe Scripps Research Institute
InventorsZeling Cai, Jonathan Sprent, Anders Brunmark, Michael Jackson, Per A. Peterson, Alain Luxembourg, Didier J. Leturcq, Ann M. Moriarty
Life of this patent
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Abstract
Materials and methods of activating T lymphocytes with specificity for particular antigenic peptides are described, as well as the use of activated T lymphocytes in vitro for the treatment of a variety of disease conditions. In particular, a synthetic antigen presenting matrix for activating T lymphocytes to a specific peptide is described.
Claims3
A synthetic antigen-presenting matrix comprising:
- a) a support comprising a fragment of a Drosophila cell;
- b) extracellular portion of at least one MHC class I molecule capable of binding to a selected peptide and being linked to the support;
- and
- c) at least one assisting molecule linked to the support such that the extracellular portions of the MHC and assisting molecules are present in sufficient numbers to activate a population of T lymphocytes specific for said peptide when the peptide is bound to the peptide binding extracellular portion of the MHC molecule;
- wherein said assisting molecule is selected from the group consisting of B7.1, B7.2, ICAM-1, ICAM-2, ICAM-3, LFA-3 and anti-CD28 antibody;
- said MHC molecule being provided by said Drosophila cell and comprising a heavy chain and a β-2 microglobulin.
3 more elements
A synthetic antigen-presenting matrix comprising:
- a) a support comprising a fragment of a Drosophila cell;
- b) extracellular portion of at least one MHC class I molecule capable of binding to a selected peptide and being linked to the support, said MHC molecule being provided by said Drosophila cell and comprising a heavy chain and a β-2 microglobulin;
- c) B-7.1 or B-7.2 molecules or a combination thereof linked to the support;
- and
- d) ICAM-1 molecules linked to the support such that the molecules are present in sufficient amount to activate a population of T-lymphocytes specific for the peptide when the peptide is bound to the peptide binding extracellular portion of the MHC molecule.
2 more elements
Dependencies are inferred from the claim text.