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OwnerDavid Henry Small, Javier Saez-Valero +1inventors
InventorsDavid Henry Small, Javier Saez-Valero, Gian Sberna
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A method for the diagnosis of Alzheimer's disease (AD) in a patient, comprising the steps of: (1) providing a sample of an appropriate body fluid from said patient; (2) detecting the presence of acetylcholinesterase (AChE) with an altered glycosylation pattern in said sample. It has been established that approximately 75-95% of the AChE in the CSF of AD patients binds to Concanavalin (Con A) or wheat germ agglutinin (WGA) but with different specificity to each. Accordingly, in order to identify the glycosylation pattern of AChE in the sample, the binding to Con A is determined, then the binding to WGA is determined, and a ratio calculated. The ratio is characteristic of the glycosylation pattern. In alternative embodiment of the invention a monoclonal antibody specific for AChE with an altered glycosylation pattern is used to detect its presence.
A method for the diagnosis of Alzheimer's disease (AD) in a patient, comprising the steps of:
An isolated and purified abnormal isoform of acetylcholinesterase (AChE) with an altered glycosylation pattern, being the amphiphilic, monomeric isoform of AChE, which has a relatively lesser affinity for Concanavalin A (Con A) and a relatively greater affinity for wheat germ agglutinin (WGA) than AChE with an unalterated glycosylation pattern.
An isolated and purified abnormal isoform of acetylcholinesterase (AChE) with an altered glycosylation pattern, being the amphiphilic, dimeric isoform of AChE, which has a relatively lesser affinity for Concanavalin A (Con A) and a relatively greater affinity for wheat germ agglutinin (WGA) than AChE with an unaltered glycosylation pattern.
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