# US 6,459,484 B1 · Scanning optical apparatus

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- Number: US 6,459,484 B1
- Document ID: US6459484B1
- Status: Granted
- Kind: B1
- Application number: 09/692,141
- Filed: 2000-10-20
- Granted: 2002-10-01
- Earliest claimed: 1999-10-21
- Assignees: Olympus Optical Co., Ltd.
- Inventors: Eiji Yokoi
- Web page: https://patentgrep.com/patent/US6459484B1

## CPC

- G01N 21/6456: {Spatial resolved fluorescence measurements; Imaging}
- G01J 3/02: Details
- G01J 3/021: {using plane or convex mirrors, parallel phase plates, or particular reflectors}
- G01J 3/0229: {using masks, aperture plates, spatial light modulators or spatial filters, e.g. reflective filters}
- G01J 3/0248: {using a sighting port, e.g. camera or human eye}
- G01J 3/14: using refracting elements, e.g. prisms (G01J3/18, G01J3/26 take precedence {prisms per se G02B5/04})
- G01J 3/36: Investigating two or more bands of a spectrum by separate detectors
- G01J 3/4406: {Fluorescence spectrometry}
- G02B 21/0076: {arrangements using fluorescence or luminescence}
- G02B 21/008: {Details of detection or image processing, including general computer control}
- G01N 2021/6441: {with two or more labels}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N)
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS

## Abstract

A scanning optical apparatus includes a light source unit, an objective lens, a scanning device, an imaging optical system, a confocal aperture, a plurality of photodetectors, a spectrum decomposing element, and a light-deflecting microelement array. The light source unit is located at the position where illumination light from the light source unit is incident through the light-deflecting microelement array on the confocal aperture. Each of light-deflecting microelements constituting the light-deflecting microelement array has deflection angles for selectively deflecting light passing through the confocal aperture toward the plurality of photodetectors and a deflection angle for deflecting the light from the light source unit toward the confocal aperture so that each of the light-deflecting microelements is capable of selecting one of these deflection angles.

## Claims

### Claim 1

A scanning optical apparatus comprising:
a light source unit;
an objective lens for collecting illumination light emitted from said light source unit on a specimen;
scanning means for relatively moving collected light and said specimen;
an imaging optical system for imaging light emanating from said specimen;
a confocal aperture placed at a position conjugate with a focal point of said objective lens;
a plurality of photodetectors for detecting the light from said specimen, passing through said confocal aperture;
a first spectrum decomposing element for spatially decomposing the light passing through said confocal aperture into a spectrum; and
a light-deflecting microelement array comprised of a plurality of light-deflecting microelements, receiving light from said first spectrum decomposing element through a first collecting optical system to deflect the light toward said photodetectors,
wherein said light source unit is located at a position where the illumination light is incident through said light-deflecting microelement array on said confocal aperture and each of said light-deflecting microelements has deflection angles for selectively deflecting the light passing through said confocal aperture toward said plurality of photodetectors and an deflection angle for deflecting the light from said light source unit toward said confocal aperture so that each of said light-deflecting microelements is capable of selecting one of these deflection angles.

### Claim 2 (depends on 1)

A scanning optical apparatus according to claim 1, wherein a second spectrum decomposing element equal in an amount of spectrum separation to said first spectrum decomposing element and a second collecting optical system for collecting light from said second spectrum decomposing element on said light-deflecting microelement array are interposed between said light source unit and said light-deflecting microelement array.

### Claim 3 (depends on 1)

A scanning optical apparatus according to claim 1, wherein a single-mode fiber is interposed between said light source unit and said second spectrum decomposing element.

### Claim 4 (depends on 3)

A scanning optical apparatus according to claim 3, wherein a coupling optical system for rendering light with a plurality of wavelengths incident on said single-mode fiber is provided.

### Claim 5 (depends on 2)

A scanning optical apparatus according to claim 2, wherein an optical isolator is interposed between said light source unit and said second spectrum decomposing element.

### Claim 6 (depends on 1)

A scanning optical apparatus according to claim 1, wherein the following condition is satisfied:
0.2<NA1/NA2<3
where NA1 is a numerical aperture of light passing through said confocal aperture, incident on said light-deflecting microelement array and NA2 is a numerical aperture of light incident from said light source unit on said light-deflecting microelement array.

### Claim 7 (depends on 2)

A scanning optical apparatus according to claim 2, wherein said second spectrum decomposing element is located at a position optically conjugate with said first spectrum decomposing element.

### Claim 8 (depends on 1)

A scanning optical apparatus according to claim 1, wherein a control device is provided which includes a memory section for storing individual deflection angles of said light-deflecting microelements, an information input section for inputting information of wavelengths of said light source unit and fluorescent dyes, and a control section for reproducing said individual deflection angles of said light-deflecting microelements stored from information inputted by said information input section.

### Claim 9 (depends on 1)

A scanning optical apparatus according to claim 1, wherein positions of said light-deflecting microelements on which light with wavelengths of said light source unit is incident are detected so that individual deflection angles of said light-deflecting microelements are determined on the basis of detected positional information.

### Claim 10 (depends on 1)

A scanning optical apparatus according to claim 1, wherein said plurality of photodetectors include photodetectors for detecting emission light produced from said specimen and photodetectors for detecting light reflected by said specimen.

### Claim 11 (depends on 1)

A scanning optical apparatus according to claim 1, wherein said light-deflecting microelements are mirror microelements.

### Claim 12

A scanning optical apparatus comprising:
a light source unit;
an objective lens for collecting illumination light emitted from said light source unit on a specimen;
scanning means for relatively scanning said specimen with collected illumination light;
an imaging optical system for imaging light emanating from said specimen;
a confocal aperture placed at a position conjugate with a focal point of said objective lens;
a plurality of photodetectors for detecting the light from the specimen, passing through said confocal aperture;
a dispersion element for spatially decomposing a light beam passing through said confocal aperture into a spectrum; and
wavelength selective means for receiving a part of the light beam decomposed into a spectrum to deflect said part toward any of said plurality of photodetectors,
wherein an apodization filter is interposed between said confocal aperture and said dispersion element.

### Claim 13

A scanning optical apparatus comprising:
a light source unit;
an objective lens for collecting illumination light emitted from said light source unit on a specimen;
scanning means for relatively scanning said specimen with collected illumination light;
an imaging optical system for imaging light emanating from said specimen;
a confocal aperture placed at a position conjugate with a focal point of said objective lens;
a plurality of photodetectors for detecting the light from the specimen, passing through said confocal aperture;
a dispersion element for spatially decomposing a light beam passing through said confocal aperture into a spectrum; and
a light-deflecting microelement array arranged, at least, in a direction of spectral decomposition and receiving a part of the light beam decomposed into a spectrum to deflect said part toward any of said plurality of photodetectors,
wherein individual light-deflecting microelements have a plurality of deflection angles which cause light beams to be selectively received by said plurality of photodetectors, and an apodization filter is interposed between said confocal aperture and said dispersion element.

### Claim 14 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein a collimating lens interposed between said confocal aperture and said first spectrum decomposing element, rendering a light beam passing through said confocal aperture substantially parallel and a collector lens for projecting a light beam decomposed into a spectrum by said first spectrum decomposing element on said light-deflecting microelement array are provided, together with an apodization filter for suppressing a side lobe of a spot of each wavelength formed by said collector lens, at least, with respect to a direction of spectral decomposition.

### Claim 15 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein said scanning means includes at least one light-deflecting element located at a position conjugate with a pupil position of said objective lens, and a scanning direction with said light-deflecting element is equal to a direction of spectral decomposition.

### Claim 16 (depends on 15)

A scanning optical apparatus according to claim 15, wherein said scanning means includes two adjacent scanning mirrors and one of said scanning mirrors is located at a position conjugate with the pupil position of said objective lens.

### Claim 17 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein an apodization region is changed in a plane perpendicular to an optical axis in association with said scanning means.

### Claim 18 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein an apodization filter includes a spatial optical modulator in which amplitude modulation is possible.

### Claim 19 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein an apodization filter is constructed so that an amplitude of a light beam is modulated only in a direction of spectral decomposition.

### Claim 20 (depends on 12, 13)

A scanning optical apparatus according to claim 12 or 13, wherein an input section for inputting information of objective lenses, a memory section for storing an amplitude profile of an apodization filter and a displacement of an optical axis involved in a scan, and a control section for controlling a change of an apodization region, on the basis of said input information, in association with said scanning means are provided.

### Claim 21 (depends on 18)

A scanning optical apparatus according to claim 18, wherein an axial-displacement detecting means for detecting a displacement of an optical axis involved in a scan is interposed between said scanning means and said first spectrum decomposing element so that an apodization region is changed in association with said scanning means, on the basis of information secured by said detecting means.

### Claim 22

A scanning optical apparatus comprising:
a light source unit;
an objective lens for collecting illumination light emitted from said laser light source unit on a specimen;
scanning means for relatively scanning said specimen with collected illumination light;
an imaging optical system for imaging light emanating from said specimen;
a confocal aperture placed at a position conjugate with a focal point of said objective lens;
a plurality of photodetectors for detecting the light from said specimen, passing through said confocal aperture;
a dispersion element for spatially decomposing a light beam passing through said confocal aperture into a spectrum; and
wavelength selective means for receiving a part of the light beam decomposed into a spectrum to deflect said part toward any of said plurality of photodetectors,
wherein an amplitude distribution of illumination light incident on a pupil of said objective lens is a substantial Gaussian distribution and a beam diameter defined as 1/e is smaller than a pupil diameter of said objective lens.