Bovine cells expressing adenovirus essential functions for propagation of recombinant adenoviral vectors
AssigneeUniversity of Saskatchewan
InventorsSuresh Kumar Tikoo, Lorne A. Babiuk, Police Seshidhar Reddy
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Abstract
The invention provides cell lines capable of supporting the replication of a defective recombinant virus vector. In one aspect, bovine cell lines expressing adenovirus E1 functions are provided. The cell lines are useful for the propagation of adenovirus vectors with mutations and/or deletions in E1 and other essential regions of the adenovirus genome.
Claims33
A recombinant fetal reitna cell of bovine origin that expresses a human E1 adenovirus gene, wherein the cell is permissive for the replication of a replication-defective recombinant bovine adenovirus vector having a mutation in the E1 gene region.
A recombinant fetal retina cell of bovine origin that expresses a human adenovirus E1 gene and wherein the cell comprises a replication-defective recombinant bovine adenovirus vector having a deletion in the E1 gene region, wherein said cell is permissive for the growth of said replication-defective bovine adenovirus vector.
A method for propagating a recombinant, replication-defective bovine adenovirus wherein said method does not produce recombinant, replication-competent bovine adenovirus, comprising growing a replication-effective bovine adenovirus vector, wherein said vector comprises a mutation in the E1 gene region, in a fetal retina cell of bovine origin that expresses a hu E1 gene and which is permissive for the growth of said replication-defective bovine adenovirus vector.
Dependencies are inferred from the claim text.