Back to results
US 6,458,557 B1Granted
Enhancing growth in gram-positive microorganisms using formate supplementation and inactivation of formate-associated transport proteins
AssigneeGenencor International, Inc.
InventorsBrian Miller, Maria Diaz-Torres
Life of this patent
Estimating the term…
Abstract
The present invention relates to the formate transport system in gram-positive microorganisms and provides methods for the production of products in a gram-positive microorganism. The present invention also provides the nucleic acid and amino acid sequences of FTAP 1 and FTAP 2 which are associated with formate transport.
Claims19
A method for producing a product in a gram-positive microorganism comprising:
- a) obtaining a gram-positive microorganism capable of producing the product;
- and
- b) culturing said microorganism in the presence of formate and under conditions suitable for production of said product,
- wherein said gram-positive microorganism is transformed with an introduced polynucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 3, and a polynucleotide sequence having at least 85% identity to SEQ ID NO:
- 1 or, SEQ ID NO:
- 3 wherein said polynucleotide sequence encodes a formate transporter associated protein (FTAP).
5 more elements
A method of producing a protein in a Bacillus host cell comprising the steps of:
- a) transforming a Bacillus host cell with a polynucleotide sequence encoding a formate transport associated protein having an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4 and a sequence at least 90% identical to SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- and
- b) culturing said Bacillus host cell in the presence of formate under conditions suitable for the production of said protein.
4 more elements
Dependencies are inferred from the claim text.