GA 20-oxidase gene sequences
AssigneeLong Ashton Research Station
InventorsTheodor Lange, Jan E. Graebe, Peter Hedden, Andrew Phillips
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Abstract
The invention relates to the molecular cloning and expression of a gibberellin (GA) 20-oxidase gene and its use, for example in transgenic plants. Aspects of the invention include recombinant DNA which encodes a polypeptide exhibiting GA 20-oxidase activity, a recombinant polypeptide exhibiting GA 20-oxidase activity, and transgenic plants which express a GA 20-oxidase gene or reverse GA 20-oxidase sequences.
Claims10
A method of preparing a DNA sequence encoding a polypeptide exhibiting GA 20-oxidase activity, said DNA sequence comprising (a) a nucleotide sequence which encodes a polypeptide exhibiting GA 20-oxidase activity having the amino acid sequence of SEQ ID No 2, 4 or 6, or (b) a nucleotide sequence which hybridizes with the nucleotide sequence of (a) or its complement under hybridizing conditions and encodes a polypeptide exhibiting GA 20-oxidase activity, said method comprising preparing a cDNA library from a suitable source organism, screening said library by means of an antibody against GA 20-oxidase or part of its amino acid sequence, or by testing for catalytic activity characteristic of the GA 20-oxidase, and preparing said DNA sequence encoding the polypeptide exhibiting GA 20-oxidase activity based on the screening results.
A recombinant polypeptide exhibiting GA 20-oxidase activity, said polypeptide comprising the amino acid sequence of SEQ ID No. 2, 4 or 6.
A recombinant polypeptide exhibiting GA 20-oxidase activity, said polypeptide being encoded by (a) a nucleotide sequence encoding the amino acid sequence of SEQ ID No 2, 4 or 6, or (b) a nucleotide sequence which hybridizes with the nucleotide sequence of (a) or its complement under hybridizing conditions.
Dependencies are inferred from the claim text.