Liquid processing method making use of pipette device and apparatus for same

A liquid processing method comprising using a pipette device which sucks a liquid containing a target high molecular substance via a smaller diameter section immersed in a liquid, of a hollow cylinder detachably set in a sucking port or a discharging port of a liquid sucking/discharging line from inside of a vessel to a larger diameter section thereof having a capacity larger than a capacity of a vessel in which a liquid is accommodated, and via an intermediate section of the hollow cylinder provided between the smaller diameter section and the larger diameter section and having a diameter smaller than at least that of the larger diameter section, in such a manner that a final liquid surface of the liquid passing through the hollow cylinder always reaches a substantially central portion of the intermediate section, and then transfers the liquid containing the target high molecular substance to a target substance processing position, comprising the steps of:

• adhering the magnetic particles absorbing or bonding to said sucked target high molecular substances onto an inner wall of the intermediate section of said hollow cylinder and separating the high molecular substance, by means of a magnetic force removably applied externally to only the substantially central portion of the intermediate section of said cylinder,
• performing steps selected from the group consisting of capturing, extracting, isolating, amplifying, labeling, and measuring said target high molecular substance only with a cylinder positioned in said liquid sucking/discharging line, a magnetic force, and at least one type of magnetic particles.

A liquid processing method comprising using a pipette device which sucks a liquid containing a target high molecular substance via a smaller diameter section immersed in a liquid of a hollow cylinder detachably set in a sucking port or a discharging port of a liquid sucking/discharging line from inside of a vessel to a larger diameter section thereof having a capacity larger than a capacity of a vessel in which a liquid is accommodated, and via an intermediate diameter section of the hollow cylinder provided between the smaller diameter section and the larger diameter section and having a diameter smaller than at least that of the larger diameter section, in such a manner that a final liquid surface of the liquid passing through the hollow cylinder always reaches a substantially central portion of the intermediate section, and then transfers the liquid containing the target high molecular substance to a target substance processing position, comprising the steps of:

• adhering the magnetic particles absorbing or bonding to said sucked target high molecular substance, onto an inner wall of the intermediate section of said hollow cylinder and separating the target high molecular substance by a means selected from the group consisting of a means using a magnetic force removably applied externally to only the substantially central portion of the intermediate section of said cylinder and a means using a filter set, and
• the steps of separating, taking out, pipetting, clarifying, condensing, diluting said target high molecular substance and/or steps for capturing, extracting, isolating, amplifying, labeling, and measuring the substance are performed in a single liquid sucking/discharging line, wherein working spaces in the single liquid sucking/discharging line are separated from each other.

A liquid processing method comprising using a pipette device which sucks a liquid containing a target high molecular substance via a smaller diameter section immersed in a liquid, of a hollow cylinder detachably set in a sucking port or a discharging port of a liquid sucking/discharging line from inside of a vessel to a larger diameter section thereof having a capacity larger than a capacity of a vessel in which a liquid is accommodated, and via an intermediate diameter section of the hollow cylinder provided between the smaller diameter section and the larger diameter section and having a diameter smaller than at least that of the larger diameter section, in such a manner that a final liquid surface of the liquid passing through the hollow cylinder always reaches a substantially central portion of the intermediate section, and then transfers the liquid containing the target high molecular substance to a target substance processing position, comprising the steps of:

• adhering the magnetic particles absorbing or bonding to said target high molecular substance onto an inner wall of the intermediate section of said cylinder by means of a magnetic force removably applied externally to only the substantially central portion of the intermediate section of said cylinder,
• agitating and mixing magnetic particles absorbed in said cylinder with a reagent or cleaning water, under conditions in which the steps of sucking and discharging liquid are continuously executed in the liquid sucking/discharging line and for times sufficient to agitate and mix the liquid with the magnetic particles, and
• controlling the temperature required for said target high molecular substance, reaction of said reagent or amplification of the target high molecular substance by transferring the reaction liquid or a liquid to be amplified into a thermostatic vessel maintaining said cylinder at a constant temperature.

A liquid processing method comprising using a pipette device which sucks a liquid containing a target high molecular substance via a smaller diameter section immersed in a liquid, of a hollow cylinder detachably set in a sucking port or a discharging port of a liquid sucking/discharging line from inside of a vessel to a larger diameter section thereof having a capacity larger than a capacity of a vessel in which a liquid is accommodated, and via an intermediate diameter section of the hollow cylinder provided between the smaller diameter section and the larger diameter section and having a diameter smaller than at least that of the larger diameter section, in such a manner that a final liquid surface of the liquid passing through the hollow cylinder always reaches a substantially central portion of the intermediate section, and then transfers the liquid containing the target high molecular substance to a target substance processing position, comprising the steps of:

• adhering the magnetic particles absorbing or bonding to said target high molecular substance onto an inner wall of the liquid passing section of said hollow cylinder and separating the target high molecular substance by a means selected from the group consisting of a magnetic force removably applied externally to only the substantially central portion of the intermediate section of said cylinder and a filter set, and
• controlling the sucking/discharging in said hollow cylinder.