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US 6,455,312 B1Granted
Regeneration system for grape and uses thereof
AssigneeUniversity of Florida
InventorsDennis J. Gray, Jayasankar Subramanian, Richard E. Litz
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Abstract
The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.
Claims3
A method of producing a perennial grape embryogenic culture, said method comprising the steps of:
- (a) visually identifying an embryogenic cell or an embryogenic cell mass from a first grape explant culture, wherein said visually identified embryogenic cell or cells of said embryogenic cell mass are identified as having a white to pale yellow color;
- (b) transferring said visually identified embryogenic cell or embryogenic cell mass of step (a) to a second culture;
- (c) allowing said transferred embryogenic cell or embryogenic cell mass of step (b) to divide into additional embryogenic cells or embryogenic cell masses;
- (d) visually identifying an embryogenic cell or embryogenic cell mass of step (c), wherein said visually identified embryogenic cell or cells of said embryogenic cell mass of step (c) are identified as having a white to pale yellow color;
- and
- (e) transferring said visually identified embryogenic cell or embryogenic cell mass of step (d) to a third culture thereby producing a perennial grape embryogenic culture.
3 more elements
Dependencies are inferred from the claim text.