Cell culture apparatus and method for culturing cells
AssigneeBioCrystal, Ltd.
InventorEmilio Barbera-Guillem
Life of this patent
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Abstract
Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; two membranes, preferably each being gas permeable, which are securedly sealed in a leak-proof selaing to a frame in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.
Claims64
A cell culture apparatus comprising:
- a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein the at least one aperture is resealable;
- two membranes, wherein each membrane comprises a gas permeable membrane, wherein the membranes are securedly sealed in a leak-proof sealing to the frame in forming a culture chamber between the two membranes and the frame, and wherein the membranes are of a sufficient optical transparency and clarity so as to permit observation of a cell culture.
A cell culture apparatus comprising:
- a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein the at least one aperture is resealable;
- two membranes, wherein at least one of the membranes comprises a gas permeable membrane, wherein the membranes are securedly sealed in a leak-proof sealing to the frame in forming multiple culture chambers between the two membranes and the frame, wherein each of the multiple culture chambers is accessible by at least one resealable aperture, and wherein the membranes are of a sufficient optical transparency and clarity so as to permit observation of a cell culture.
A cell culture apparatus comprising:
- a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein the at least one aperture is resealable;
- two membranes, wherein at least one of the membranes comprises a gas permeable membrane, wherein the membranes are securedly sealed in a leak-proof sealing to the frame in forming a culture chamber between the two membranes and the frame, and wherein the membranes are of a sufficient optical transparency and clarity so as to permit observation of a cell culture;
- and
- a magnetic sheet extended over and detachably secured to the cell culture apparatus in a face to face manner, wherein the magnetic sheet is in a position selected from the group consisting of close proximity to, and in contact with, a membrane which forms the culture chamber of the cell culture apparatus.
A bioreactor comprising a plurality of cell culture apparatuses, wherein a fluid passageway is provided between the plurality of cell culture apparatuses, wherein the fluid passageway allows for fluid flow communication between culture chambers of cell culture apparatuses connected by the fluid passageway, and wherein a cell culture apparatus comprises:
- a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein the at least one aperture is resealable;
- two membranes, wherein at least one of the membranes comprises a gas permeable membrane, wherein the membranes are securedly sealed in a leak-proof sealing to the frame in forming a culture chamber between the two membranes and the frame, and wherein the membranes are of a sufficient optical transparency and clarity so as to permit observation of a cell culture.
A method of introducing a recombinant DNA molecule into cells cultured in a cell culture apparatus, the method. comprising:
- (a) mixing a transfection reagent with the recombinant DNA molecule comprising a vector in forming a mixture comprising the recombinant DNA molecule;
- (b) mixing the mixture comprising the recombinant DNA molecule with tissue culture medium in forming medium comprising the recombinant DNA molecule;
- (c) introducing the medium comprising the recombinant DNA molecule into the culture chamber so as to contact the cultured cells in the cell culture apparatus;
- and
- (d) incubating the medium comprising the recombinant DNA molecule in contact with the cultured cells in promoting introduction of the recombinant DNA molecule into cultured cells in the culture chamber;
- wherein the cell culture apparatus comprises
- (i) a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein the at least one aperture is resealable, and
- (ii) two membranes, wherein at least one of the membranes comprises a gas permeable membrane, wherein the membranes are securedly sealed in a leak-proof sealing to the frame in forming a cell culture chamber between the two membranes and the frame, and wherein the membranes are of a sufficient optical transparency and clarity so as to permit observation of the cell culture.
Dependencies are inferred from the claim text.