Methods and compositions for controlling biofilm development

A biofilm removing or inhibiting composition comprising an amount of a compound selected from the group consisting of blockers of N-(3-oxododecanoly) L-homoserine lactone (OdDHL), and mixtures of blockers of OdDHL and analogs of butyryl L-homo-serine lactone (BHL), and a vehicle or carrier, wherein the amount of the compound is effective to remove or disrupt a bacterial biofilm or inhibit normal biofilm formation wherein the OdDHL blocker has the following structure:

• wherein
• R1-R21 are selected from the group consisting of H, C1-C4 alkyl group, OH, NH2, SH and a halogen, wherein the halogen is selected from the group consisting of fluorine, chlorine, bromine, and iodine;
• R22 and R23 are selected from a halogen, H, S, N, NH, and O
• R24-R28 is H or a halogen, and
• X1 and X2 are selected from O, S, H2 or any combination of H plus one halogen or two halogens when one or more R groups is substituted, and at least one of R1-R21 is a halogen, or the alkylene chain of the molecule contains a sulfur in the chain.

A biofilm removing or inhibiting composition comprising an amount of a compound selected from the group consisting of analogs of butyryl L-homo-serine lactone (BHL), and mixtures of analogs of BHL and blockers of N-(3-oxododecanoly) L-homoserine lactone (OdDHL), and a vehicle or carrier, wherein the amount of the compound is effective to remove or disrupt a bacterial biofilm or inhibit normal biofilm formation wherein the analog of BHL has the following structure:

• wherein
• R1-R7 are selected from the group consisting of H, C1-C4 alkyl group, OH, NH2, SH and a halogen, wherein the halogen is selected from the group consisting of fluorine, chlorine, bromine, and iodine;
• R22 and R23 are selected from H, S, N, NH, and O
• R24-R28 is H or a halogen, and
• X is selected from O, S, H2 or any combination of H plus one halogen H plus OH or NH2, a double bonded O, NH, S, or two halogens when one or more R groups is substituted, and at least one of R1-R7 is a halogen, or the alkylene chain of the molecule contains a sulfur in the chain.

A biofilm removing or inhibiting composition comprising an amount of a compound selected from the group consisting of blockers of N-(3-oxododecanoly) L-homoserine lactone (OdDHL), analogs of butyryl L-homo-serine lactone (BHL), and mixtures thereof, and a vehicle or carrier, wherein the amount of the compound is effective to remove or disrupt a bacterial biofilm or inhibit normal biofilm formation, wherein the OdDHL blocker has the structure of formula I and the BHL analog has the structure of formula II:

• wherein
• R1-R21 are selected from the group consisting of H, C1-C4 alkyl group, OH, NH2, SH and a halogen, wherein the halogen is selected from the group consisting of fluorine, chlorine, bromine, and iodine;
• R22 and R23 are selected from a halogen, H, S, N, NH, and O
• R24-R28 is H or a halogen, and
• X, X1 and X2 are selected from O, S, H2 or any combination of H plus one halogen or two halogens when one or more R groups is substituted,
• provided that when the biofilm comprises an OdDHL blocker alone or a BHL analog alone, at least one of R1-R21 is a halogen, or the alkylene chain of the molecule contains a sulfur in the chain.