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US 6,451,569 B1Granted
Synthetic molecules that specifically react with target sequences
AssigneeThe Regents of the University of California
InventorsRoger Y. Tsien, B. Albert Griffin
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Abstract
The present invention features biarsenical molecules. Target sequences that specifically react with the biarsenical molecules are also included. The present invention also features kits that include biarsenical molecules and target sequences. Tetraarsenical molecules are also featured in the invention.
Claims21
A method of cross-linking two bonding partners comprising
- a) providing a tetraarsenical molecule capable of specifically reacting with a first and a second target sequence;
- and
- b) contacting said tetraarsenical molecule with at least two bonding partners under conditions effective for said tetraarsenical molecule to specifically react with said first and said second target sequences, said first bonding partner comprising said first target sequence, said second bonding partner comprising said second target sequence,
- wherein said first and said second target sequence comprise one or more cysteines capable of specifically reacting with a biarsenical molecule of general formula,
1 more element
A method of purifying a bonding partner, comprising:
- a) providing a bonding partner, comprising a target sequence, wherein said target sequence comprises one or more cysteines capable of specifically reacting with a biarsenical molecule, of general formula,
- and tautomers, anhydrides, and salts thereof;
- wherein
- each X1 or X2, independently, is Cl, Br, I, ORa, or SRa, or
- X1 and X2 together with the arsenic atom form a ring having the formula:
- Ra is H, C1-C4 alkyl, CH2 CH2OH, CH2COOH or CN;
- Z is 1,2-ethanediyl, 1,2, benzenediyl, 4-methyl-1,2-benzenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;
- Y1 and Y2, independently, are H or CH3;
- or
- Y1 and Y2, together form a ring such that the biarsenical molecule has the formula:
- where M is O, S, CH2, C(CH3)2, or NH;
- R1 and R2, independently, are ORa, OAc, NRaRb, or H;
- R3 and R4, independently, are H, F, Cl, Br, I, ORa, or Ra;
- or
- R1 together with R3, or R2 together with R4, or both, form a ring in which
- (i) one of R1 or R3 is C2-C3 alkyl and the other is NRa and
- (ii) one of R2 and R4 is C2-C3 alkyl and the other is NRa;
- Rb is H, C1-C4 alkyl, CH2CH2OH, CH2COOH or CN;
- Q is CRaRb, CRaORb, C═O, or a spirolactone having the formula:
- wherein the spiro linkage is formed at C1,
- b) contacting said bonding partner with said biarsenical molecule, wherein said biarsenical molecule is coupled to a solid phase,
- c) eluting said bonding partner from said biarsenical molecule by contacting said biarsenical molecule with a dithiol.
19 more elements
Dependencies are inferred from the claim text.