Releasing embedding media from tissue specimens
AssigneeBioGenex Laboratories
InventorsGuangrong Zhan, Krishan L. Kalra, Sheng-Hui Su, Taiying Chen
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Abstract
Releasing the embedding medium from an embedded histochemically reactive tissue specimen is provided by contacting the embedded tissue specimen with a releasing composition under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.
Claims20
A method for releasing the embedding medium from an embedded histochemically reactive tissue specimen, said method comprising contacting said embedded tissue specimen with a releasing composition comprising a non-polar organic solvent, a polar organic solvent, a surfactant, and water, under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.
An automated method for releasing the embedding medium from an embedded histochemically reactive tissue specimen, said automated method comprising
- (a) providing an apparatus comprising means for performing a predetermined sequence of operations under a predetermined set of conditions for histochemical analysis which includes at least the step of releasing the embedding medium from an embedded histochemically reactive tissue specimen;
- and
- (b) contacting said embedded tissue specimen under the control of said apparatus with a releasing composition comprising a non-polar organic solvent, a polar organic solvent, a surfactant, and water, under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.
Dependencies are inferred from the claim text.