Pta LDHA double mutant Escherichia coli SS373 and the method of producing succinic acid therefrom
AssigneeKorea Institute of Science and Technology
InventorsJao Go Pan, Soo An Shin, Chan Kyu Park, Pil Kim, Dong Eun Chang, Jae Eun Kim
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Abstract
This invention relates to a mutant Escherichia coli SS373 and the production of succinic acid by using the above strain. In detail, a novel E. coli SS373 (W3110 pta::Tn10 ldhA::Km) with the deficiency in the acetate and lactate forming pathways was constructed by genetic engineering technique. An aerobically grown SS373 was then cultured by means of the anaerobic condition shift during the succinate producing stage, which resulted in the efficient production of succinic acid with a higher yield.
Claims4
A phosphotransacetylase (pta) lactate dehydrogenase (ldhA) double mutant E. coli SS373 (KCTC 0506BP) which lacks the ability to produce lactate and acetate.
A method of making a succinic acid-producing E. coli strain, wherein insertional mutations in the pta and ldhA genes result in a strain deficient in the production of lactate and acetate, and enhanced production of succinic acid.
Dependencies are inferred from the claim text.