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US 6,448,055 B1Granted
Δ9-desaturase gene
AssigneeSuntory Limited
InventorsSakayu Shimizu, Michihiko Kobayashi
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AssigneeSuntory Limited
InventorsSakayu Shimizu, Michihiko Kobayashi
Estimating the term…
Genomic DNA and cDNA encoding Δ9-desaturase from a microorganism belonging to the subgenus Mortierella of the genus Mortierella, an expression vector for expression thereof, and a transformant are disclosed. A method for producing Δ9-desaturase by use of a gene encoding the enzyme is also disclosed. Introduction of the Δ9-desaturase gene of the present invention into an unsaturated fatty acid producing cell can enhance conversion into palmitoleic acid or oleic acid, starting materials for unsaturated fatty acids, and can increase the production of unsaturated fatty acids. By combining a gene for cytochrome b5 or a gene for cytochrome b5 reductase, constituents of the microsomal electron transport system, with the Δ9-desaturase of the present invention, more efficient production can be expected. Δ9-desaturase can also be produced with high efficiency by a recombinant DNA technology.
An isolated or purified DNA sequence encoding the amino acid sequence of Seq. ID No. 4.
An isolated or purified DNA sequence of Seq. ID No. 3.
An isolated or purified DNA sequence comprising a DNA sequence of Seq. ID No. 5.
A purified or isolated polypeptide comprising the amino acid sequence of Seq. ID No. 4.
A purified or isolated polypeptide with an activity of desaturating a Δ9-position of fatty acid, wherein said polypeptide is derived from a microorganism Mortierella alpina.
An isolated or purified DNA sequence derived from a microorganism Mortierella alpina, which encodes Δ9-desaturase.
Dependencies are inferred from the claim text.