PgsA
AssigneeSmithKline Beecham Corporation
InventorsDavid John Holmes, Chantal Myriam Petit, Christopher Michael Traini, Richard Lloyd Warren
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Abstract
The invention provides pgsA polypeptides and polynucleotides encoding pgsA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pgsA polypeptides to screen for antibacterial compounds.
Claims22
An isolated polynucleotide comprising a nucleic acid sequence that is identical to SEQ ID NO:1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- wherein the nucleic acid sequence is not genomic DNA and wherein the nucleic acid sequence detects Staphylococcus aureus by hybridization.
An isolated polynucleotide, comprising a nucleic acid sequence which hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- and, wherein the nucleic acid sequence is identical to SEQ ID NO:1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- wherein the nucleic acid sequence is not genomic DNA and wherein the nucleic acid sequence detects Staphylococcus aureus by hybridization.
An isolated polynucleotide comprising a nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the nucleic acid sequence is not genomic DNA.
An isolated polynucleotide comprising a nucleic acid sequence which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, wherein the nucleic acid sequence is not genomic DNA.
An isolated polynucleotide segment comprising a nucleic acid sequence comprising SEQ ID NO:1, wherein the nucleic acid sequence is not genomic DNA.
Dependencies are inferred from the claim text.