Protein with human choriogonadotropin inhibitory effect
AssigneeCedars-Sinai Medical Center
InventorsGlenn D. Braunstein, Song-Guang Ren
Life of this patent
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Abstract
A substance having an inhibitory effect on the production of human choriogonadotropin (hCG) is described, which comprises a protein isolated from human decidual cells with a preliminary amino acid analysis and a molecular weight of about 7,000 to about 10,000 daltons, and useful for use in inhibiting production of hCG in vivo or in vitro, may be measured as an indication of a cause of hCG inhibition, or used in the treatment of hCG-secreting tumors.
Claims8
A method for evaluating inhibition of human chorionic gonadotropin (hCG) production of a subject's trophoblast cells comprising the steps of isolating a regulatory polypeptide
- a) which inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro;
- b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;
- c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro;
- and
- d) which exhibits a molecular weight of about 7,000 to about 10,000 daltons, as determined by ultrafiltration and gel exclusion chromatography;
- from human decidual cells of said subject;
- and quantifying the amount of the regulatory polypeptide produced in vitro by said subject's human decidual cells.
A method for evaluating inhibition of chorionic gonadotropin (hCG) production of a subject's trophoblast cells comprising the steps of isolating a regulatory polypeptide
- a) which inhibits, in a dose dependent matter, the production of hCG by human trophoblasts in vitro;
- b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;
- c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro;
- and
- d) which exhibits a molecular weight of about 7,000 to about 10,000 Daltons, as determined by ultrafiltration and gel exclusion chromatography;
- from human decidual cells of said subject;
- and quantifying the amount of the regulatory polypeptide produced in vivo by said subject's human decidual cells.
Dependencies are inferred from the claim text.