DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
AssigneeCaliper Technologies Corp.
InventorsMorten Jensen, J. Wallace Parce
Life of this patent
Estimating the term…
Abstract
A method is provided for identifying components of a mixture by labeling the individual components with fluorescent agents having different fluorescence lifetimes. The components are subsequently separated, fluorescent labels detected and their lifetimes measured. Based on the measured fluorescent lifetimes, the components of mixtures of small organic molecules, polymers, peptides, saccharides and nucleic acids can be identified.
Claims24
A method of distinguishing between a plurality of fluorescent species, wherein each of said fluorescent species has a fluorescence emission, said emission having a characteristic fluorescence lifetime, said method comprising:
- (a) electrokinetically transporting each of said fluorescent species through a microfluidic
- (b) detecting each of said fluorescent species in said channel;
- and,
- (c) identifying each of said fluorescent species by measuring said characteristic fluorescence lifetime.
A method of distinguishing between a plurality of fluorescent species, wherein each of said fluorescent species has a fluorescence emission, said fluorescence emission having a characteristic fluorescence lifetime, the method comprising:
- (a) electrokinetically separating each of said fluorescent species;
- (b) detecting each of said fluorescent species;
- and,
- (c) identifying each of said fluorescent species by measuring said characteristic fluorescence lifetime.
Dependencies are inferred from the claim text.