Back to results
US 6,444,992 B1Granted
High throughput microscopy
AssigneeTrellis Bioscience, LLC
InventorsLawrence M. Kauvar, John Sedat
Life of this patent
Estimating the term…
Abstract
An apparatus for high resolution, high throughput, three-dimensional fluorescence microscopy with a rotatable circular disc for mounting samples to be viewed and multiple CCDs and beam splitters as detectors for the fluorescence emission.
Claims16
An apparatus for high resolution, three dimensional, fluorescence microscopy including:
- an objective lens,
- a microscope stage for receiving and positioning a means for containing one or more samples to be viewed through said lens,
- a light source providing light from the UV to the near IR wavelengths,
- an excitation light pathway from said light source through said lens to said sample which includes means for directing light from said light source to said lens and means for selecting a desired wave length for excitation,
- an emission light pathway from said sample through said objective lens to at least one CCD which includes a light path selector and an emission filter means, and
- a computer imaging means connected to said CCD,
- wherein the means for containing one or more samples is a rotatable circular glass disc,
- wherein said rotatable circular disc is divided into a multiplicity of sample-receiving areas, and
- wherein said areas are defined by hydrophobic barriers, or
- wherein the sample-receiving areas are defined by an adhered adhesive.
7 more elements
An apparatus for high resolution, three dimensional, fluorescence microscopy applications including:
- an objective lens,
- a microscope stage for receiving and positioning a means for containing one or more samples to be viewed through said lens,
- a light source providing light from the UV to the near IR wavelengths,
- an excitation light pathway from said light source through said lens to said sample which includes means for directing light from said light source to said lens and means for selecting a desired wave length for excitation,
- an emission light pathway from said sample through said objective lens to multiple CCDs which pathway includes a light path selector, an emission filter means, and multiple beam splitters which cause the same primary fluorescent light to be imaged simultaneously on multiple CCD detectors, and
- a computer imaging means connected to said CCDs.
3 more elements
Dependencies are inferred from the claim text.