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AssigneeDanisco A/S
InventorsIain A. Donaldson, Kirsten Bojsen, Kirsten Jorgensen, Morten Jorsboe
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A selection method for selecting from a population of plant cells one or more genetically transformed plant cells is described. In the method, the population of plant cells includes selectable genetically transformed plant cells and possible non-transformed plant cells. Each of the selectable genetically transformed plant cells comprises a first expressible nucleotide sequence and optionally a second expressible nucleotide sequence. In the method, a component or a metabolic derivative thereof when present in a low concentration in a medium is a nutrient for both the selectable genetically transformed plant cells and the non-transformed plant cells. In the method, the component or the metabolic derivative thereof when present in a high concentration in a medium is toxic to the non-transformed plant cells. The first nucleotide sequence codes for a gene product having glucosamine-6-phosphate deaminase activity which is capable of converting the component or the metabolic derivative thereof when present in a high concentration in a medium to a nutrient for the selectable genetically transformed plant cells. The method includes the step of introducing the population of plant cells to a medium, wherein the medium includes a high concentration of the component or the metabolic derivative thereof. In the method, the component or the metabolic derivative thereof is a source of both carbohydrate and nitrogen for the selectable genetically transformed plant cells.
A selection method for selecting genetically transformed plant cells from a population of plant cells, comprising:
A composition comprising a population of transformed and non-transformed plant cells, said transformed plant cells being transformed with an expressible nucleotide sequence having at least 75% identity to SEQ ID NO:
A construct for genetically transforming a non-transformed plant cell to produce a selectable genetically transformed plant cell, said construct comprising:
A transgenic plant produced from a cell transformed with an expressible nucleotide sequence having at least 75% identity to SEQ ID NO:
An E. coli strain selected from the group consisting of NCIMB 40852, NCIMB 40853, and NCIMB 40854.
An isolated or purified polynucleotide having a sequence which comprises SEQ ID No:
Dependencies are inferred from the claim text.