Acyl CoA: cholesterol acyltransferase related nucleic acid sequences
AssigneeCalgene, Inc.
InventorsMichael W. Lassner, Diane M. Ruezinsky
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Abstract
By this invention, novel nucleic acid sequences encoding for acyl-CoA: cholesterol acyltransferase (ACAT) related proteins are provided, wherein ACAT-like protein is active in the formation of a sterol ester and/or triacylylgycerol from a fatty acyl-CoA and sterol and/or diacylglycerol substrates. Also considered are amino acid and nucleic acid sequences obtainable from ACAT-like nucleic acid sequences and the use of such sequences to provide transgenic host cells capable of producing sterol esters and/or triacylglycerols.
Claims32
An isolated DNA molecule encoding a protein comprising an amino acid sequence of SEQ ID NO:
- 2.
A recombinant DNA construct encoding a protein having a sequence comprising an amino acid sequence of SEQ ID NO:2.
A plant cell having a DNA construct encoding a protein comprising an amino acid sequence of SEQ ID NO:2.
A method of modifying the lipid composition in a plant cell, said method comprising:
- transforming a plant cell with a recombinant DNA construct having a DNA sequence encoding a protein comprising an amino acid sequence of SEQ ID NO:2, and
- growing said cell under conditions wherein transcription of said DNA sequence is initiated,
- whereby said lipid composition is modified.
A method of modifying the lipid composition in a host cell, said method comprising:
- transforming a host cell with a DNA construct comprising as operably associated components in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in said host cell, a DNA sequence encoding a protein having an amino acid sequence of SEQ ID NO:
- 2, and a transcription termination sequence, and
- growing said cell under conditions wherein transcription of said DNA sequence is initiated,
- whereby said lipid composition is modified.
An isolated DNA molecule comprising a nucleic acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.