Hydroperoxide lyase gene from maize and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsJon Duvick, Jacob Gilliam
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Abstract
Compositions and methods for enhancing disease resistance, and modulating levels of flavor molecules in plants are provided. The compositions comprise nucleotide sequences encoding maize HPL genes. The methods comprise expressing hydroperoxide lyase (HPL) genes in plants, plant cells, and plant tissues. Expression cassettes comprising the HPL sequences of the invention are provided, as well as transformed plant cells, plant tissues, and plants.
Claims17
An isolated nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1;
- and
- (b) a nucleotide sequence set forth in SEQ ID NO:2.
A method for modulating expression of hydroperoxide lyase in a transformed plant relative to an untransformed plant, said method comprising transforming a plant cell with at least one nucleotide sequence encoding a maize hydroperoxide lyase protein, and regenerating a stably transformed plant from said transformed plant cell, wherein said stably transformed plant has modulated hydroperoxide lyase expression relative to said untransformed plant, and wherein said nucleotide sequence is operably linked to a promoter that drives expression in said transformed plant, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1;
- and
- (b) a nucleotide sequence set forth in SEQ ID NO:2.
A transformed plant cell having stably incorporated into its genome at least one nucleotide sequence encoding a hydroperoxide lyase protein, said nucleotide sequence operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1;
- and
- (b) a nucleotide sequence set forth in SEQ ID NO:2.
A transformed plant having stably incorporated into its genome an expression cassette comprising at least one nucleotide sequence encoding a hydroperoxide lyase protein, said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1;
- and
- (b) a nucleotide sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.