Recombinant human papillomavirus vaccine expressed in transgenic plants
AssigneeGenomine, Inc.
InventorsUlk Sohn, Hong Gil Nam, Deok Hoon Park, Kuk Hyun Kim
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Abstract
The oral vaccine of the present invention containing the HPV like particles is produced in edible transgenic plants and then administered through the consumption of the edible portion of those plants or after the purification of the particles. A DNA sequence encoding one of the HPV capsid proteins is isolated, inserted into the plasmid which comprises a plant-specific promoter and a gene for a selection marker, and then transferred to plant cells to give transgenic plants. Since the gene is expressed in a portion of the plants, the oral vaccine of the present invention can be produced in an inexpensive manner and administered in a simple method.
Claims7
A transgenic plant comprising a DNA sequence encoding a human papillomavirus (HPV) capsid protein, wherein said plant assembles the HPV capsid protein into antigenic HPV like particles.
A prophylactic or therapeutic vaccine comprising HPV like particles, wherein the vaccine is produced as a pharmaceutically active ingredient by a method for producing an antigenic composition containing HPV like particles comprising the steps of:
- 1) constructing a plasmid vector comprising a DNA sequence encoding an HPV capsid protein operably linked to a plant-specific promoter and a gene encoding a selection marker;
- 2) transforming a plant cell or tissue with the plasmid vector of step 1;
- 3) inducing a callus formation from the transformed plant cell or tissue of step 2;
- 4) inducing a root formation in root-inducing medium, adjusting to soil, and regenerating a transgenic plant;
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- 5) recovering HPV like particles from the transgenic plant.
Dependencies are inferred from the claim text.