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AssigneesCuris, Inc., Biogen, Inc.
InventorsR. Blake Pepinsky, Darren P. Baker, Dingyi Wen, Kevin P. Williams, Ellen A. Garber, Frederick R. Taylor, Alphonse Galdes, Jeffrey Porter
Estimating the term…
Hydrophobically-modified proteins and methods of making them are described. A hydrophobic moiety is attached to a surface amino acid residue of the protein. The hydrophobic moiety can be a lipid or a peptide. Alternatively, the protein can be derivatized by a wide variety of chemical reactions that append a hydrophobic structure to the protein. The preferred protein is of mammalian origin and is selected from the group consisting of Sonic, Indian, and Desert hedgehog. The hydrophobic moiety is used as a convenient tether to which may be attached a vesicle such as a cell membrane, liposome, or micelle.
An isolated protein comprising an N-terminal amino acid and a C-terminal amino acid, wherein the protein is selected from the group consisting of:
An isolated protein comprising an N-terminal amino acid and a C-terminal amino acid, wherein the protein is selected from the group consisting of:
An isolated hedgehog protein modified with one or more lipophilic moieties with the proviso that, in the instance wherein the hedgehog polypeptide is the mature N-terminal proteolytic fragment of a hedgehog protein, the lipophilic moiety is other than a sterol at the C-terminal residue, which hedgehog polypeptide binds to patched and has an amino acid sequence at least 80 percent identical to a hedgehog amino acid sequence selected from the group consisting of SEQ ID No:5, SEQ ID No:6, SEQ ID No:7, SEQ ID No:8, SEQ ID No:9, SEQ ID No:10, SEQ ID No:11, SEQ ID No:12, SEQ ID No:13 and SEQ ID No:14, or an extracellular fragment thereof.
An isolated protein comprising a hedgehog amino acid sequence derivatived with at least one fatty acid moiety, which hedgehog amino acid sequence binds to patched and is at least 80 percent identical to a hedgehog amino acid sequence selected from the group consisting of SEQ ID No:5, SEQ ID No:6, SEQ ID No:7, SEQ ID No:8, SEQ ID No:9, SEQ ID No:10, SEQ ID No:11, SEQ ID No:12, SEQ ID No:13 and SEQ ID No:14, or an extracellular fragment thereof.
A method for modifying a physico-chemical property of a protein, comprising introducing at least one hydrophobic moiety to an N-terminal cysteine of the protein or to a functional equivalent of the N-terminal cysteine, wherein the protein is a hedgehog protein.
Dependencies are inferred from the claim text.