Method of isolating a peptide which immunologically mimics microbial carbohydrates including group B streptococcal carbohydrates and the use thereof in a vaccine
AssigneeThe Research and Development Institute, Inc.
InventorSeth H. Pincus
Life of this patent
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Abstract
This invention relates to new vaccines against microorganisms based on antigenically mimetic peptides. The invention also relates to methods of discovering such mimetic peptides by first screening peptide-display phage libraries with antibodies against the microbial carbohydrates(s) of interest to locate antigenically mimetic peptides. Vaccines against Group B Streptococcus, or Streptococcus Agalactiae, are preferably produced using this method.
Claims13
A peptide that induces an immunoprotective response against Group B Streptococci Type III, wherein said peptide specifically binds to an antibody that specifically binds to Group B Streptococci Type III capsular polysaccharide.
A peptide having a sequence selected from the group consisting of FDTGAFDPDWPA (SEQ ID NO:
- 1), FDTGAFDPDWPAC (SEQ ID NO:
- 2), WENWMMMGNA (SEQ ID NO:
- 3), WENWMMGNAC (SEQ ID NO. 4) that induce an immunoprotective response against Group B Strep.
A peptide that induces an immunoprotective response against Group B Streptococci Type III capsular polysaccharide, the peptide being produced by the process of:
- (a) contacting a phage peptide display library with one or more protective antibodies that specifically bind to a Group B Streptococci Type III capsular polysaccharide;
- (b) isolating one or more phage from the phage peptide display library that bind to said one or more protective antibodies;
- and
- (c) identifying those peptides from the phage peptide display library that specifically bind to said one or more protective antibodies.
Dependencies are inferred from the claim text.