Reticulocyte containing complete blood control
AssigneeResearch & Diagnostic Systems, Inc.
InventorAlan M. Johnson
Life of this patent
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Abstract
A control composition, and method of preparing a control composition, that includes stabilized, maturation-arrested reticulocytes, and a complete blood count base including mature erythrocytes, stabilized white cells and stabilized platelets, or analogs thereof.
Claims17
A control composition comprising a predetermined concentration of stabilized reticulocytes, and a complete blood count base including mature erythrocytes, stabilized white cells or analogs thereof, and stabilized platelets or analogs thereof, wherein the reticulocytes are maturation-arrested to provide a desired maturation profile.
A ready-to-use kit comprising a plurality of control compositions wherein at least one of the control compositions include a predetermined concentration of reticulocytes, mature red blood cells, stabilized white blood cells or analogs thereof, and stabilized platelets or analogs thereof, wherein the reticulocytes are maturation-arrested to provide a desired maturation profile.
A method of preparing a control composition, the method comprising the steps of:
- (1) harvesting an enriched population of porcine reticulocytes;
- (2) treating the reticulocytes with a suitable medium to arrest further maturation;
- (3) stabilizing the harvested, arrested reticulocytes;
- and (4) preparing a control composition comprising a pre-determined concentration of stabilized, maturation-arrested reticulocytes, red blood cells, white blood cells or analogs thereof, and platelets or analogs thereof.
A control composition comprising a predetermined concentration of a reticulocyte component, and a complete blood count base including mature erythrocytes, stabilized white cells or analogs thereof, and stabilized platelets or analogs thereof and wherein the reticulocyte component is provided at concentrations from about 0.5% to about 25% of the total red blood cell count, wherein the reticulocytes are maturation-arrested to provide a desired maturation profile.
A control composition comprising a predetermined concentration of a reticulocyte component, and a complete blood count base including mature erythrocytes, stabilized white cells or analogs thereof, and stabilized platelets or analogs thereof and wherein the reticulocyte component is provided at concentrations from about 0.5% to about 15% of the total red blood cell count, wherein the reticulocytes are maturation-arrested to provide a desired maturation profile.
A control composition comprising a predetermined concentration of a reticulocyte component, and a complete blood count base including mature erythrocytes, stabilized white cells or analogs thereof, and stabilized platelets or analogs thereof and wherein the reticulocyte component is provided at concentrations from about 1% to about 12% of the total red blood cell count, wherein the reticulocytes are maturation-arrested to provide a desired maturation profile.
Dependencies are inferred from the claim text.